Introduction: Colorectal cancer (CRC) is the second leading cause of death in developed countries, mainly as a consequence metastatic spread. Secreted proteins are essential to communicate cells with tumor- microenvironment, favoring tumor progression and metastasis. The conditioned medium or cellular secretome, including exosomes, has demonstrated be rich source effectors biomarkers metastatiz...

With the improved secreted protein prediction approach and comprehensive data sources, including Swiss-Prot, TrEMBL, RefSeq, Ensembl and CBI-Gene, we have constructed secretomes of human, mouse and rat, with a total of 18 152 secreted proteins. All the entries are ranked according to the prediction confidence. They were further annotated via a proteome annotation pipeline that we developed. We ...

Helminth parasites secrete several types of biomolecules to ensure their entry and survival in hosts. The proteins secreted the extracellular environment participate pathogenesis anthelmintic immune responses. aim this work was identify functionally annotate excretory/secretory (ES) monogenean ectoparasite Rhabdosynochus viridisi through bioinformatic approaches. A total 1655 putative ES were i...

It is degraded to free triglycerides and fatty acids by the secreted phosphatases of plant family (sPLA2s). Plants have very few sPLA2s. Plant sPLA2s' molecular, biochemical, catalytic properties are being studied. Three-dimensional structures also included when comparing two groups. Glycine max used as a benchmark for various organisms, including any herbal plants small animals. In addition, t...

American Foulbrood is a severe disease affecting larvae of honeybee Apis mellifera, causing significant decrease in the honeybee population, beekeeping industries and agricultural production. In spite of its importance, little is known about the virulence factors secreted by Paenibacillus larvae during larval infection. The aim of the present work was to perform a first approach to the identifi...

Twenty mucin genes have been identified and classified in two groups (encoding secreted membrane-bound proteins). Secreted mucins participate mucus formation by assembling a 3-dimensional network via oligomerization, whereas are anchored to the outer membrane mediating extracellular interactions cell signaling. Both associated with carcinogenesis progression epithelial cancers, therefore consid...

Chlamydia trachomatis is an obligate intracellular pathogen that replicates in a vacuole termed the inclusion. Many of the interactions of chlamydiae with the host cell are dependent upon bacterial protein synthesis and presumably exposure of these proteins to the cytosol. Because of the dearth of genetic tools for chlamydiae, previous studies examining secreted proteins required the use of het...

The secreted proteins from a cell constitute a natural biologic library that can offer significant insight into human health and disease. Discovering new secreted proteins from cells is bounded by the limitations of traditional separation and detection tools to physically fractionate and analyze samples. Here, we present a new method to systematically identify bioactive cell-secreted proteins t...

Arrays of subnanoliter wells (nanowells) provide a useful system to isolate single cells and analyze their secreted proteins. Two general approaches have emerged: one that uses open arrays and local capture of secreted proteins, and a second (called microengraving) that relies on closed arrays to capture secreted proteins on a solid substrate, which is subsequently removed from the array. Howev...

Analysis of proteins in biological samples opens up the possibility of discovering new markers of toxicity. The liver is one of the primary targets of drug-induced toxicity, and it also secretes many plasma proteins, which can be measured clinically. Most of the plasma proteins produced by the liver are secreted by hepatocytes, but there is little information regarding the protein profile secre...