A Piscirickettsia salmonis was isolated from the brain of a diseased coho salmon during an outbreak in Southern Chile. The isolate, AS-98, showed similar genetic, biochemical, serological and virulence characteristics to the type strain LF-89. The brain might represent an important residence site for P.salmonis. Piscirickettsia salmonis is an endemic and widely distributed pathogen among cultur...

Vibrio parahaemolyticus is a pathogenic Gram-negative halophilic bacterium, a natural inhabitant of the marine environment and can be found in crabs, shrimps, fish, oysters, mussels and other seafoods. We areisolate and study the hemolytic activity of the pathogenic V. parahaemolyticus by different blood samples (human, rabbit and rat). Visualization of hemolysis of red blood cells in agar plat...

17 -Ethynylestradiol (EE), a major constituent of many oral contraceptives, inactivated the testosterone 6 -hydroxylation activity of purified P450 3A4 reconstituted with phospholipid and NADPH-cytochrome P450 reductase in a mechanismbased manner. The inactivation of P450 3A4 followed pseudo first order kinetics and was dependent on NADPH. The values for the KI and kinact were 18 M and 0.04 min...

The metabotropic glutamate (mGlu) receptors mGlu1 and mGlu5 mediate distinct inositol 1,4,5-trisphosphate (IP3) and Ca signaling patterns, governed in part by differential mechanisms of feedback regulation after activation. Single cell imaging has shown that mGlu1 receptors initiate sustained elevations in IP3 and Ca 2 , which are sensitive to agonist concentration. In contrast, mGlu5 receptors...

Cereal Chern. 65(3):215-222 Zein, the major alcohol-soluble protein of corn endosperm, was extracted with 55% 2-propanol. It was then fractionated by preparative reversed-phase (CIs) high-performance liquid chromatography (RPH PLC) with an acetonitrile-triOuoroacetic acid gradient. From 15 to 17 peaks resulted for native or reduced and alkylated (R-A) zeins. Sodium dodecyl sulfate-polyacrylamid...

Low high-density lipoprotein-cholesterol (HDL-c) is an important risk factor of coronary artery disease (CAD). Optimum therapy for raising HDL-c is still not available. Identification of novel HDL-raising agents would produce a major impact on CAD. In this study, we have identified a potent (IC50 24 nM) and selective peroxisome proliferator-activated receptor (PPAR ) agonist, 2-methyl-2-(4-{3-[...

Protein kinase B (Akt) plays an essential role in the actions of insulin, cytokines and growth factors, although the substrates for PKB that are relevant to many of its actions require identification. In this study we report the identification of p122RhoGAP, a GTPase activating protein selective for RhoA and rodent homologue of the tumour suppressor gene Deleted in Liver Cancer (DLC1), as a nov...

Using single particle electron cryomicroscopy that does not impose icosahedral averaging, we determined the structure of the entire infectious Salmonella phage Epsilon151, including both icosahedral and non-icosahedral components. At least three layers of condensed viral DNA were observed to pack in coaxial coils with local 25 Å hexagonal inter-strand spacing. At one of the fivefold vertices, a...

Bacteriophage λ protein phosphatase (λPP) is a member of a large family of metal-containing phosphoesterases, including purple acid phosphatase, protein serine/threonine phosphatases, 5′-nucleotidase, and DNA repair enzymes such as Mre11. λPP can be activated several-fold by various divalent metal ions, with Mn2+ and Ni2+ providing the most significant activation. Despite the extensive characte...

Background: Flos Lonicerae japonicae (FLJ; Kinginka) is the dried flowers and buds of the Japanese honeysuckle Lonicera japonica Thunberg. FLJ has been used as a Japanese Kampo medicine to treat infectious and inflammatory diseases. However, it is not clear which constituent of FLJ is responsible for its pharmacological effects. Methods: FLJ was extracted with methanol and fractionated by hydro...