The main goal of this study was to describe the method of the synthesis of the dodecyl-, tetradecyl-, hexadecyl- and octadecyl-propylene glycol emulsifiers in the presence of selected anionic and nonionic surfactants. Acyl propylene glycol emulsifiers were produced by esterification of propane-1,2-diol (propylene glycol, PG) with C12:0-C18:0 fatty acids in the presence of anionic sodium dodecyl...

The reaction between [PtCl2(DMSO)2] and L-cysteine (L-Cys) has been investigated in the presence of micelles of sodium dodecyl sulfate (SDS)--as a model for biological membranes. Additionally, the inhibitory effect of [PtCl2(DMSO)2] on the Na+,K+-ATPase activity and its partial prevention with 10 mM L-Cys were demonstrated. The interaction of L-Cys with [PtCl2(DMSO)2] resulted in the formation ...

oxidation reaction of indole to isatin was performed in the presence of fe3o4 nanoparticles (as catalyst) and commercial grade perhydrol (as oxidant). magnetite nanoparticles were synthesized via chemical precipitation method and characterized by xrd and tem techniques. the efficiency of reaction was obtained from gc analysis of the mixture. the catalyst was easily separated from the reaction m...

Human factor VIII was purified from cryoprecipitate and subjected to enzymatic degradation with plasmin, trypsin, chymotrypsin, and thrombin. The reactions were monitored by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and by measurement of four biologic properties, namely, procoagulant activity, ristocetin cofuctor activity, precipitation by heterologous antibody, and neutra...

Diffusion-controlled dissolution and precipitation reactions occur in many biological systems and some non-stirred in vitro systems. Previous studies have shown that differences in the diffusion rates of the ions involved in a dissolution/precipitation reaction can produce significant effects on the rate and course of the reaction. We report here results of a study that show inter-diffusion of ...

A series of Ce1 ́xCoxO2 ́y precursors were prepared by homogeneous precipitation using urea as a precipitant. The Co/CeO2 catalysts obtained from the Ce1 ́xCoxO2 ́y precursors were used for the steam reforming of ethanol to produce hydrogen. Co ions could enter the CeO2 lattices to form Ce1 ́xCoxO2 ́y mixed oxides at x ď 0.2 using the homogeneous precipitation (hp) method. CeO2 was an excellent suppo...

Purification of a soluble cytochrome c6 from the unicellular green alga Scenedesmus obliquus by a simple and rapid method is described. The purification procedure includes ammonium sulfate precipitation and non-denaturating PAGE. The N-terminal sequence of the first 20 amino acids was determined and shows 85% similarity and 75% identity to the sequence of cytochrome c6 from the green alga Monor...

We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chr...

Histidine decarboxylase was purified 800-fold from the kidneys of thyroxine-treated mice. The purification procedure included precipitation of protein from a crude supernatant after heating it to 55 degrees C at pH 5.5, fractionation with (NH4)2SO4, phosphocellulose column chromatography, chromatofocusing, DEAE-Sepharose column chromatography, gel filtration on Sephacryl S-300 and preparative p...

A new procedure for the partial purification of Mg2+-dependent, N-ethylmaleimide-sensitive phosphatidate phosphohydrolase (Mg2+-PAP; EC 3.1.3.4) from rat liver cytosol is described, using protein precipitation with MgCl2, gel filtration on Sephacryl S-400, chromatography on DEAE-cellulose and affinity chromatography on calmodulin-agarose. From the parallel change in staining intensity and in th...