Nowadays the photonic crystal hydrogel materials have shown great promise in the detection of different chemical analytes, including creatinine, glucose, metal ions and so on. In this paper, we developed a novel three-dimensional photonic crystal hydrogel, which was hydrolyzed by sodium hydroxide (NaOH) and immobilized with butyrylcholinesterase (BuChE) by 1-(3-Dimethylaminopropyl)-3-ethylcarbo...

We give an axiomatic foundation to the updating rule proposed by [Sarin, R. and Wakker, P. P. (1998). Revealed likelihood and knightian uncertainty. Journal of Risk and Uncertainty 16(3):223-250.] for CEU preferences. This rule is dynamically consistent but non-consequentialist, since forgone consequences are relevant for conditioning. Whereas it does not work universally, but only when counter...

Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase that does not require a template, is found in thymocytes and marrow Tlymphocytes normally [1,2]. Shortly after these discoveries, McCaffrey et al. [3] described the presence of this enzyme in the leukemic cells of patients with acute lymphoblastic leukemia (ALL) and Sarin et al. [4] discovered that some patients with chronic m...

U police are investigating allegations made by a former Royal Air Force (RAF) serviceman that experiments at Porton Down Chemical and Biological Defence Establishment in which he participated left him with a long-term debilitating illness. Gordon Bell attributes his injuries directly to the experiments, and is reported to have welcomed the Wiltshire Police’s decision to investigate his claims. ...

The protein kinase C (PKC) signaling pathway has been associated with modulation of N-metyl-D-aspartate receptor activity, motor behavior, learning, and memory, all of which are severely impaired in organophosphate (OP) intoxication. Nevertheless, the role of PKC in OP intoxication is largely unknown. The present study attempted to characterize alterations in the immunoreactivity levels of PKC ...

Departments of Chemistry (M.R.R.) and Biochemistry and Biophysics (A.C.H., C.D.F., M.R.R), University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; U.S. Army Medical Research Institute of Chemical Defense (T.C.O., D.M.C.), Aberdeen Proving Ground, Maryland; Human BioMolecular Research Institute (M.M., J.R.C.), San Diego, California; Department of Molecular Pharmacology (M.W., C...

Although recent innovations in transient plant systems have enabled gram quantities of proteins in 1-2 weeks, very few have been translated into applications due to technical challenges and high downstream processing costs. Here we report high-level production, using a Nicotiana benthamiana/p19 system, of an engineered recombinant human acetylcholinesterase (rAChE) that is highly stable in a mi...

In the present paper we show a comprehensive in vitro, ex vivo and in vivo study on hydrolytic detoxification of nerve agent and pesticide OPs (organophosphates) catalysed by purified hBChE (human butyrylcholinesterase) in combination with novel non-pyridinium oxime reactivators. We identified TAB2OH (2-trimethylammonio-6-hydroxybenzaldehyde oxime) as an efficient reactivator of OP-hBChE conjug...