The effects of the addition of three inorganic salts, namely, NaCl, Na(2)SO(4), and Na(3)PO(4), on the liquid-liquid (L-L) phase diagram of aqueous solutions containing the model ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate, [bmim][BF(4)], were investigated. All three inorganic salts trigger salting-out effects, leading to significant upward shifts of the L-L demixing temperatures...

1. Conditions under which gelatin may be salted out into two liquid layers at 35 degrees were studied. 2. The equilibria governing the amounts and composition of the layers salted out with sodium chloride are found to accord with the requirements of the phase rule for the quaternary system gelatin-sodium chloride-hydrogen ion-water. 3. So far, soaps and gelatin are found to be surprisingly simi...

Although the crystallographic structure of the bacterial porin OmpF has been known for a decade, the physical mechanisms of its ionic selectivity are still under investigation. We address this issue in a series of experiments with varied pH, salt concentrations, inverted salt gradient, and charged and uncharged lipids. Measuring reversal potential, we show that OmpF selectivity (traditionally r...

as it appears from the results of clinical laboratory investigations was discussed in former papers (7, 10, 11). Our studies were based on the comparative investigation of normal and pathologic sera by means of electrophoresis and the salting-out technic. These observations, in accordance with the findings of other workers (4), indicated a correlation of the results obtainable by the two method...

Simultaneous brine thawing/salting process was applied as an alternative to traditional pile salting process using 51 frozen Iberian hams. The effect of this type of salting process on endogenous enzyme activity and sensory quality of Iberian dry-cured hams was analysed. The frozen hams were simultaneously thawed and salted with saturated brine, with and without vacuum pulses, and were compared...

We have isolated an enzyme, phenylalanine ammonia-lyase (EC 4.3.1.5.), from the rootlets of Zea mays var. СЕ-IV. After partial purification by fractional salting out with ammonium sulfate and gel chromatography on Sephadex G-100, we obtained a preparation of a 40-fold greater specific activity than that of the crude homogenate. We hbve determined the ap­ parent Michaelis constant and the activa...

Abstract This work aimed to obtain aspartic proteases of industrial and biotechnological interest from the stomach crevalle jack fish (Caranx hippos). In order do so, a crude extract (CE) was obtained subjected partial purification by salting-out, which resulted in enzyme (EE) obtainment. EE were characterized physicochemically means zymogram. addition, effect chemical agents on their activity ...

Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11 of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application of this procedure resulted in a 95.1-fold purification of LT with a yield of 19.9% as determined by rabbit ileal loop assay. The final LT preparation s...