The mouse c-Ki-ras protooncogene promoter contains a homopurine-homopyrimidine domain that exhibits S1 nuclease sensitivity in vitro. We have studied the structure of this DNA region in a supercoiled state using a number of chemical probes for non-B DNA conformations including diethyl pyrocarbonate, osmium tetroxide, chloroacetaldehyde, and dimethyl sulfate. The results demonstrate that two typ...

The apolipoprotein E (ApoE) gene has been linked to maladies such as hypercholesterolemia, CNS injury and disease. In this study, we present evidence that, in addition to the known transcript (ApoE S1) that translates into ApoE, there are three additional transcripts in mice. Two of these transcripts, ApoE S2 and ApoE S3, which are predicted to be transmembrane proteins, are transcribed from th...

Poly-A+ mRNA from Xenopus laevis oocytes, partially enriched for r-protein coding capacity has been used as starting material for preparing a cDNA bank in plasmid pBR322. The clones containing sequences specific for r-proteins have been selected by translation of the complementary mRNAs. Clones for six different r-proteins have been identified and utilized as probes for studying their genomic o...

The basis for our score is a feature vector corresponding to a given input. In our case, the input is a sequence to be classified, and the features are going to be the output of certain tools (see workflow in Figure 1) we feed with the input sequence. In the following, we describe the two distinct steps necessary for the computation: as a prerequisite, we first establish distributions from our ...

Table B: One-tailed p-values for F̂ST based on WGS data. †With the exception of the compairison between MKK and MLA in 2014, all p-values are Monte Carlo estimates based on 1000 random permutations as described in Table A. The p-value for MKK and MLA in 2014 is exact, since there were fewer than 1000 possible permutations given nMKK = 4 parasite samples from MKK, and nMLA = 8 parasite samples fr...

n k − is the rate of constitutive degradation of lateral signaling. Lateral signaling is mediated by complexes of LIN-12-cyto with LAG-1 transcription factor. Silencing of lateral signaling occurs due to proteosomal degradation of LIN-12-cyto [1]. The mean half-life of proteins susceptible to proteolysis has been measured to be approximately 43 minutes [2]. Therefore, the reference value of par...

In this communication, we describe several features of the D. melanogaster gene which codes for ribosomal protein 49 (rp49). Nucleotide sequence analysis in conjunction with primer extension and S1 nuclease protection experiments show that the structure of the rp49 gene consists of a 102 bp 5' exon, a single 59 bp intron, and a 420 bp 3' exon, encoding a total of 132 amino acids. The rp49 gene ...