A method is described for identifying different genotypes of hepatitis C virus (HCV) by restriction endonuclease cleavage of sequences amplified by PCR from the 5' non-coding region. Using the enzymes HaeIII-RsaI and HinfI-MvaI, followed by cleavage with BstU1 or ScrFI, it was possible to identify and distinguish HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5 and 6. The method was used to investiga...

A functional centromere located on a small DNA restriction fragment from Saccharomyces cerevisiae was identified as CEN14 by integrating centromere-adjacent DNA plus the URA3 gene by homologous recombination into the yeast genome and then by localizing the URA3 gene to chromosome XIV by standard tetrad analysis. DNA sequence analysis revealed that CEN14 possesses sequences (elements I, II, and ...

Two DNA duplexes of identical sequence and 35 nt in length were synthesized by an original and a highly improved version of phosphoramidite chemistry. By base composition analysis, DNA synthesized by improved chemistry (termed DMTS-imp) contained no detectable modified bases while DNA synthesized by the original chemistry (termed DMTS-std) had a large number of modifications. Under optimal reac...

genetic improvement programs may improve disease resistance in animal production. the best-characterized genetic control of disease resistance and immune response in animals is the one associated with the major histocompatibility complex (mhc). the ovine lymphocyte antigenof drb1 gene encodes cell surface glycoproteins that initiate immune responses by presenting processed antigenic peptides to...

Source/Description: A 1.5 kb Apal-Apal fragment, containing 5' upstream sequences of the human CYP2E gene (1). Polymorphism: Pstl detects a two allele polymorphism with bands at 6.1 kb (Cl) or 3.9 and 2.2 kb (C2). Rsal detects a two allele polymorphism with bands at 1.4 and 0.5 kb (Cl) or 1.9 kb (C2). These two polymorphisms are in complete linkage disequilibrium with each other. Other Comments...

Human cytochrome P4502E1 (CYP2E1) is a well-conserved xenobiotic-metabolizing enzyme expressed in liver, kidney, nasal mucosa, brain, lung, and other tissues. CYP2E1 is inducible by ethanol, acetone, and other low-molecular weight substrates and may mediate development of chemically-mediated cancers. CYP2E1 polymorphisms alter the transcriptional activity of the gene. This study was conducted i...