The objective of this study was to optimize the operating parameters of the International Rice Research Institute (IRRI) Test Tube rice mill. The goal was to minimize the milling duration required to attain a milling degree equivalent to a 12% brown rice kernel mass loss. Operating parameters including brown rice mass, milling duration, tube oscillation frequency, and test tube size were invest...

This study was planned in order to make good the lack of information about population-based data on the prevalence of brucellosis in the Kayseri region. This cross-sectional research was performed on 1850 individuals living in the rural regionaround Kayseri. People aged 15-85 in 9 districts in this rural areas were interviewed and blood samples were screened using the Rose Bengal Agglutination ...

The epidemiology and control of Brucella melitensis in Malta was analysed using herd test data made available by the Veterinary Service of Malta. The eradication scheme commenced in 1987 with the introduction of a test and slaughter scheme using the Rose Bengal test. Herds registered with Malta Dairy Products Limited (MDP) showed a herd prevalence of 23% in 1987 which fell to less than 1.5% by ...

In order to evaluate the usefulness of a peripheral blood PCR assay in the posttreatment follow-up of brucellosis, a cohort of 30 patients was studied by means of blood cultures, rose Bengal, seroagglutination, Coombs' antibrucella tests, and PCR assay at the time of diagnosis, at the end of treatment, and 2, 4, and 6 months later. Of the 29 patients whose PCR assays were initially positive, 28...

We investigated the seroprevalence and risk factors for Brucella seropositivity in cattle in Jordan. The sera from 671 cows were randomly collected from 62 herds. The antibodies against Brucella were detected using a Rose Bengal plate test and indirect ELISA. A structured questionnaire was used to collect information on the cattle herds' health and management. A multiple logistic regression mod...

Bovine brucellosis, an important bacterial zoonosis, is usually associated with intensive systems of production. A cross-sectional study was conducted in western Ethiopia to determine the seroprevalence of bovine brucellosis in cattle undertraditional extensive husbandry. Sera collected from 1,152 cattle originating from 164 herds were screened, using the Rose Bengal test, and all positive sera...

bengal, asparagus agar (Allen, 1951), asparagus agar plus 1:80,000 crystal violet, and asparagus agar plus 1:15,000 rose bengal. The addition of dyes decreased the number of contaminants but also appeared to inhibit the development of the rhizobia. All plates were examined daily for 2 weeks. Smears of colonies which resembled rhizobia were examined. Pure cultures were obtained by the use of bot...

We have examined the actions of histidine-specific reagents on potassium channels in squid giant axons. External application of 20-500 microM diethylpyrocarbonate (DEP) slowed the opening of potassium channels with little or no effect on closing rates. Sodium channels were not affected by these low external concentrations of DEP. Internal application of up to 2 mM DEP had no effect on potassium...

Quantitative recoveries of bacteria added to a number of soils were obtained using fluorescein isothiocyanate (FITC) as a stain. Enzyme fluorescence, using fluorescein diacetate or dibutyrate, could not be adapted for the routine counting of metabolizing organisms in soil. Staining with rose bengal gave recoveries of 60-80%. Soil-extract dilution plates revealed 4 X 107 bacteria/g in the 0-10 c...

BACKGROUND Melitococcosis is one of the most widespread zoonoses worldwide. In the period from 2009 to 2013, comprehensive melitococcosis testing was conducted in the Republic of Croatia. METHODS AND RESULTS During the testing, the Rose Bengal test was applied to 344019 blood samples of sheep and goats, and positive reactions were confirmed in 1143 (0.3%) of samples. The complement fixation t...