MCM-41 mesoporous silica is synthesized based on a self assembly method, using a quaternary ammonium template, CTAB for the adsorption of sulfur compounds from model oil (1.0 mmol/l DBT in dodecane solution). Then the adsorption capability of MCM-41 assembled on the surface of bacterium Rhodococcus erythropolis IGTS8 is examined regarding the improvement of the biodesulfurization process of...

Carbendazim (methyl 1H-benzimidazol-2-yl carbamate) is one of the most widely used fungicides in agriculture worldwide, but has been reported to have adverse effects on animal health and ecosystem function. A highly efficient carbendazim-degrading bacterium (strain dj1-11) was isolated from carbendazim-contaminated soil samples via enrichment culture. Strain dj1-11 was identified as Rhodococcus...

Bacteria containing mycolic acids in their cell envelope are often recalcitrant to lysis, so extracting DNA of sufficient quality for third-generation sequencing and high-fidelity genome assembly requires optimization, even when using commercial kits with protocols hard-to-lyse bacteria. We benchmarked three spin-column-based against a classical extraction method employing lysozyme, proteinase ...

Microbes capable of degrading pollutant such as 1,4-dioxane were selected from the seawater samples of four coastal areas of Okinawa located in the most southern islands of Japan using the developed method and a seawater-charcoal perfusion apparatus. Specifically, aerobic culture in the apparatus led to the selection of candidate 1,4-dioxane degraders. Among these, strain RM-31 and KY-3 exhibit...

Strain DCL14, which is able to grow on limonene as a sole source of carbon and energy, was isolated from a freshwater sediment sample. This organism was identified as a strain of Rhodococcus erythropolis by chemotaxonomic and genetic studies. R. erythropolis DCL14 also assimilated the terpenes limonene-1,2-epoxide, limonene-1,2-diol, carveol, carvone, and (-)-menthol, while perillyl alcohol was...

The biochemical characterization of the muconate and the chloromuconate cycloisomerases of the chlorophenol-utilizing Rhodococcus erythropolis strain 1CP previously indicated that efficient chloromuconate conversion among the gram-positive bacteria might have evolved independently of that among gram-negative bacteria. Based on sequences of the N terminus and of tryptic peptides of the muconate ...

background sulfur oxides released from the burning of oil causes severe environmental pollution. the sulfur can be removed via the 4s pathway in biodesulfurization (bds). immobilization approaches have been developed to prevent cell contamination of oil during the bds process. objectives the encapsulation of rhodococcus erythropolis r1 in calcium alginate beads was studied in order to enhance c...

Chiral 2-methylpiperidine (2-MPI) is an important building block that has potential for applications in pharmaceuticals and pesticides. In this study, we observed the hydrolase Arthrobacter sp. K5 exhibits high (S)-selectivity toward rac-N-pivaloyl-2-MPI to yield (S)-2-MPI with 80.2% enantiomeric excess (ee) a 38.2% conversion. The hydrolase, which was identified by analyses of partial amino ac...