In previous work, we showed that coinoculating Rhizobium leguminosarum bv. viciae 128C53 and Bacillus simplex 30N-5 onto Pisum sativum L. roots resulted in better nodulation and increased plant growth. We now expand this research to include another alpha-rhizobial species as well as a beta-rhizobium, Burkholderia tuberum STM678. We first determined whether the rhizobia were compatible with B. s...

to study the including rhizobium treatment (strain numbers of 133, 116 & combination of them) along with 100% nitrogen fertilizer application and a check (no inoculation & no fertilizer) on certain agronomical and physiological traits of three common bean cultivars (viz. bahman, darkhshan & sayiad) a field experiment was performed at research station of university of tehran (karaj-iran) in 2010...

rRNA gene sequencing and PCR assays indicated that 215 isolates of root nodule bacteria from two Mimosa species at three sites in Costa Rica belonged to the genera Burkholderia, Cupriavidus, and Rhizobium. This is the first report of Cupriavidus sp. nodule symbionts for Mimosa populations within their native geographic range in the neotropics. Burkholderia spp. predominated among samples from M...

The current classification of the rhizobia (root-nodule symbionts) assigns them to six genera. It is strongly influenced by the small subunit (16S, SSU) rRNA molecular phylogeny, but such single-gene phylogenies may not reflect the evolution of the genome as a whole. To test this, parts of the atpD and recA genes have been sequenced for 25 type strains within the alpha-Proteobacteria, represent...

Periplasmic cyclic beta-glucans of Rhizobium species provide important functions during plant infection and hypo-osmotic adaptation. In Sinorhizobium meliloti (also known as Rhizobium meliloti), these molecules are highly modified with phosphoglycerol and succinyl substituents. We have previously identified an S. meliloti Tn5 insertion mutant, S9, which is specifically impaired in its ability t...

Rhizobium spp. are found in soil. They are both free-living and found symbiotically associated with the nodules of leguminous plants. Traditionally, studies have focused on the association of these organisms with plants in nitrogen-fixing nodules, since this is regarded as the most important role of these bacteria in the environment. Rhizobium sp. are known to possess several replicons. Some, l...

Mutualisms are key determinants of community assembly and composition, but urbanization can alter the dynamics these interactions associated effects on ecosystem functions. Legume–rhizobia mutualisms a model interaction to evaluate ecological ecosystem-level urbanization, particularly urban-driven eutrophication nitrogen (N) deposition. Here, we evaluated how affected ecology mutualism between ...

A set of conserved, or common, bacterial nodulation (nod) loci is required for host plant infection by Rhizobium melioti and other Rhizobium species. Four such genes, nodDABC, have beeii indicated in R. meliloti 021'by genetic analysis and DNA sequencing. An essential step toward Iunderstanding the function of these genes is to characterize their protein products. We used in vitro and maxicell ...

A field study was undertaken during winter (rabi) season of 2013-2014 to explore the effect of graded levels of fertilizers and bio-fertilizers on yield of chickpea, protein content in chickpea seeds and nutrient uptake by the crop. Significantly higher seed and stover yields (1854, 2722 kg ha-1, respectively) were recorded with application of fertilizer @ 100% RDF (25:50:00, kg N:P:K ha-1). Re...