Introduction: The most common cutaneous fungal infections are caused by dermatophyte fungi such as Microsporum, Trichophyton, and Epidermophyton. In this study, the epidemiologic trends predominant organisms causing dermatophytosis in Urmia, Northwest Iran, were identified.Aims objectives: To get better perception of distribution authors studied identification isolated dermatophytes from human ...

OBJECTIVES To use a molecular typing method (opa-typing) to characterise gonococcal isolates obtained from men who have sex with men (MSM) attending a genitourinary medicine (GUM) clinic in Edinburgh during 2001. To compare the results of opa-typing with A/S (auxotype/serovar) phenotyping, and with epidemiological information obtained by contact tracing. METHODS Isolates were opa-typed by a P...

Tuberculosis patients may be infected with or have disease caused by more than one Mycobacterium tuberculosis strain, usually referred to as "mixed infections." These have mainly been observed in settings with a very high tuberculosis incidence and/or high HIV prevalence. We assessed the rate of mixed infections in a population-based study in rural Vietnam, where the prevalences of both HIV and...

The present study aimed at comparing the trypanosome specific 18S-PCR-RFLP using samples stored either on Whatman filter papers (PCR-RFLP-fp) or in a commercial cell lysis and DNA protecting buffer (PCR-RFLP-pb) with the haematocrit centrifugation technique (HCT), a method widely used for the diagnosis of African Animal Trypanosomosis. Out of 411 head of cattle, 49 (11.92%) (CI=8.95-15.45) scor...

Pasteurella multocida (P. multocida), A Gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. The outer membrane of Gram-negative bacteria contains of many different protein in very high copy numbers. One of the major outer membrane, the H proteins have functional as high immunogenicity and antigenicity. In this study ...

One of the major opportunistic pathogens in patients with burn injuries is Pseudomonas aeruginosa, which causes severe infections in burned patients. The objective of the study was to examine the molecular epidemiology of P. aeruginosa colonization in the burn unit of Shahid Motahari Hospital in Tehran, Iran. Restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (...

In this Round Table, the application of several methods of molecular typing were discussed in reference to four important pathogenic fungi: Coccidioides immitis, Histoplasma capsulatum, Candida albicans and Paracoccidioides brasiliensis. Among the different methods the following were discussed: restriction fragment length polymorphisms (RFLP), single nucleotide polymorphisms, random amplified p...

Restriction fragment length polymorphism (RFLP) analysis of open reading frame 5 was developed for typing of Czech strains of porcine reproductive and respiratory syndrome virus (PRRSV). e set of restriction enzymes Acc I, Hae II and SnaB I allowed the differentiation of heterogeneous Czech strains of PRRSV clustered separately in the phylogenetic tree. e high-passage strain V-502 (164) was a...

The cervical cancer is currently the most prominent case of association between HPV and cancer. The persistent infection of the anogenital tract by a group of HPV types has been proved to be the main causal factor of cervical cancer (Bosch et al., 2002; Walboomers et al., 1999). This group of cervical cancer related types currently includes 30 to 40 types. However, not all the types in the grou...

A total of 20 skin lesion samples collected from patients with cutaneous leishmaniasis (CL) attended AL-Karamah Teaching Hospital at Wasit province. Dermal scrapings were analysed by examination of Giemsa-stained smears, by Conventional PCR and RFLP-PCR methods. The incidence rate with CL was 80% by using Giemsa-stained and 100% for both PCR and RFLP-PCR. Twelve (60%) of isolates were typed as ...