Abstract. Ermawati D, Panjono, Bintara S, Hartatik T. 2022. Diversity of partial sequence leptin gene (Exon 3) in crossbred cattle compared to GenBank database. Biodiversitas 23: 5614-5620. Genetic marker method that is often used select Single Polymorphism Nucleotide (SNP). Polymorphisms (SNP) has been found various candidate genes, one which the gene. Leptin a affects reproduction and weight....

During the last few decades, the use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in plant biotechnology and their genetics studies. There are different types of markers viz. morphological, biochemical and DNA based molecular markers. These DNA based markers are differentiates in two types first non PCR based (RFLP) and second is PCR based m...

Casein genes directly control milk protein of animals. CSN1S1 (αS1-Casein) and CSN2 (β-Casein) influence on fractions. Genetic polymorphisms gene at g.12164G>A locus g.8913C>A were identified by PCR-RFLP technique. Animal samples pure dairy goats providing PE (5 hds.), Saanen (8 hds.) their crosses Sapera (50% Saanen, 50% PE) (51 SaanPE (75% 25% (3 from IRIAP goat station. Allele frequenc...

Late blight, caused by the pathogen Phytophthora infestans, is one of the most devastating diseases of potato. Here, we describe a new single dominant resistance gene, Rpi2, from the Mexican diploid wild species Solanum pinnatisectum that confers high level and broad spectrum resistance to late blight. The Rpi2 locus confers full resistance to complex isolates of P. infestans, for which race sp...

The present study aimed at comparing the trypanosome specific 18S-PCR-RFLP using samples stored either on Whatman filter papers (PCR-RFLP-fp) or in a commercial cell lysis and DNA protecting buffer (PCR-RFLP-pb) with the haematocrit centrifugation technique (HCT), a method widely used for the diagnosis of African Animal Trypanosomosis. Out of 411 head of cattle, 49 (11.92%) (CI=8.95-15.45) scor...

Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being Fusarium graminearum the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eight primers, respectively. IGS-RFLP yielded 41 bands, 30 of which were polymorphic while ISSR produced 87 bands with 4...

in the present study the allelic polymorphisms of gh, ghr and tgfβ3 genes and its association with egg production traits were investigated. blood samples randomly were collected from breeder hens of mazandaran native fowls breeding station and transported to the laboratory in cold chain condition. dna was extracted using modified salting out method and the desired loci were amplified by specifi...

The X-chromosome, highly conserved within mammals, has been shown to contain major quantitative trait loci (QTL) for growth and fat deposition in the pig. We have discovered a BamHI polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) marker that was assigned to the porcine X-chromosome by two-point and multi-point linkage analysis following genotyping of a three-genera...

Sinh sản và xuất thịt là những tính trạng kinh tế quan trọng trong chăn nuôi. Để cải thiện di truyền này, chỉ thị phân tử đã được ứng dụng rộng rãi ở nhiều nước có hệ thống nuôi phát triển. Những nghiên cứu gần đây cho rằng gen RNF212 NCAPG liên đến khả năng sinh sức động vật vú. Trong đó, đột biến thay thế nucleotide đơn C>T trên biết làm gia tăng tỷ lệ tái tổ hợp giảm bò. Hơn nữa, tương th...

Calreticulin (CALR) mutations are included in the WHO diagnostic criteria of Myeloproliferative Neoplasm (MPN). Presence CALR mutation, aids their diagnosis by excluding reactive causes. There several molecular techniques to detect mutations; however, these require advanced equipment and technical expertise. An in-house “polymerase chain reaction-restriction fragment length polymorphism (PCR- R...