Medroxyprogesterone acetate is widely used in veterinary medicine as intravaginal dosage for the synchronization of breeding cycle in ewes and goats. The main goal of this study was to develop reverse-phase high-performance liquid chromatography method for the quantification of medroxyprogesterone acetate in veterinary vaginal sponges. A single high-performance liquid chromatography/UV isocrati...

Mammalian cell proteins, modified post-translationally by derivatives of [3H]mevalonic acid, were subjected to methylation and sulfonium salt cleavage reactions previously used to release isoprenoids from cysteine residues in yeast peptides. The labeled isoprenoid extracted into chloroform comigrated with farnesol through a series of chromatography steps including Sep-Pak C-18 fractionation, si...

styrene is an important constituent of widely used organic solvents in industries for production of various synthetic materials. the use of solid-phase extraction (spe) has grown and is a fertile technique of sample preparation as it provides better results than those produced by liquidliquid extraction. in this study, spe has been optimized, regarding sample ph, sample concentration, sample fl...

Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using Pseudomonas aeruginosa in in vitro experiments . Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg L (-1) TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples taken at 0, 6, 12, 24, 48, 72 an...

Coloradocin was isolated from a fermentation broth by adsorption onto Amberlite XAD-2. The activity was eluted in MeOH and purified by gel filtration on Shephadex LH-20, followed by liquid-liquid chromatography on diol-bonded silica gel. The last two steps in the purification of this antibiotic included reverse-phase chromatography on C18-bonded silica gel and countercurrent chromatography on a...

abstract mefenamic acid is a nonsteroidal anti-inflammatory drug (nsaid) that has analgesic,anti-infammatory and antipyretic actions. it is used to relieve mild to moderate pains. solid-phase extraction of mefenamic acid by a polymer grafted to silica gel is reported . poly allyl glycidyl ether/iminodiacetic acid-co-n, n-dimethylacrylamide was synthesized and grafted to silica gel and was used ...

separation of ten sedative- hypnotic drugs was performed by rp-hplc using mixed micellar mobile phase. effect of temperature, type and amount of organic modifier in mobile phase on efficiency (n) and asymmetry factor (b/a) showed that, the appropriate conditions for a good separation were 35°c and 7% (v/v) butanol in mobile phase.  variations of selectivity factor versus butanol concentration, ...

A simple, rapid and precise reverse phase high performance liquid chromatography method has been developed and validated for the determination of Afloqualone in tablet and serum. Chromatography was performed by Shimadzu Model LC-20 ATVP with Kromasil C-18 column 250 x 4.6 mm ID with 5  particle sizes and the column maintained at ambient temperature. The injection volume was 20l and the total ...

Radix isatidis (Banlangen) is a widely used traditional Chinese medicine for treating fever and removing toxic heat. Pharmacological studies have indicated that (R)-goitrin (epigoitrin) is one of the main constituents accounting for its antiviral activity, while (S)-goitrin (goitrin) is known as an antithyroid factor. To better control the quality of radix isatidis and its formulations, it is i...

We describe a chemical assay involving "high-pressure" liquid chromatography for the quantitative determination of three aminoglycosides: netilmicin, tobramycin, and gentamicin. The drugs are separated from serum by means of precipitation of the serum proteins with acetonitrile after dilution with a buffer. The aminoglycosides are quantitatively extracted into the supernate, which is further pu...