BACKGROUND Two meningococcal serogroup B vaccines contain Factor H binding protein (FHbp). Binding of Factor H (FH) to FHbp was thought to be specific for human or chimpanzee FH. However, in a previous study an amino acid polymorphism in rhesus macaque FH domain 6, tyrosine at position 352 (Y352) was associated with high binding to FHbp, whereas histidine at position 352 (H352) was associated w...

Although in the cow the genetic resistance to brucellosis has been previously attributed to the Slc11A1 gene encoding Nramp1 protein, none of the mutations described to date seems to be the cause. To be able to associate another polymorphism of the gene to brucellosis resistance, we characterized the gene and identified in different breeds of Bos taurus and Bos indicus, six new variants among a...

the genotypes for beta-lactoglobulin (blg) and calpastatin (cast) were determined by polymerase chain reaction (pcr) and restriction enzyme digestion and genotyped for calpain (capn) by pcr-sscp method in a native iranian breed sheep, kordi. blood samples were collected from 100 pure bred kordi sheep from kordi breeding station located in shirvan, mashhad. the extraction of genomic dna was base...

Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analysis was used to investigate variation in the ovine KAP6-3 gene (KRTAP6-3) in 383 Merino × Southdown-cross lambs from four sire-lines, and to determine whether this variation affects wool traits. Five PCR-SSCP banding patterns, representing five different nucleotide sequences, were detected, including four prev...

background: variants in the candidate genes enos, cyp11b2 and ace have been implicated as liable biomarkers that can predict complications like hypertension and preeclampsia. studies on the impact and distribution of these variants on healthy pregnancy have not been done so far in south indian or in any of the native indian population. examining these variants could lay a strong basis in unders...

The objective of this study was to investigate the effects variety and plant environment on nutritional composition, phenolic contents antioxidant activities from commercial wheat cultivars grown in Pakistan. Chemical total were measured twenty-seven spring varieties for two cropping years. Preliminary assessment strength extracts carried out by evaluation (TPC), activity (AA) free radical scav...

Heat-treated carbohydrate-rich foods may contain high levels of acrylamide (AA). Crisp bread is a significant dietary AA source in the Nordic countries. We studied whether urinary metabolites of AA could be candidate biomarkers of AA intake and internal dose in mice following dietary crisp bread administration or sc injection. The crisp bread was experimentally baked to contain three different ...

Methods Seventy-three patients with FMF and 39 patients with active systemic inflammation due to autoimmune or idiopathic inflammation were evaluated in our reference center for amyloidosis. Mutations in MEFV and the SAA-1 exon 3 variants were analyzed for their contribution to AA risk. Amyloidosis was detected in all cases by congo red staining of kidney or bowel biopsy specimens and AA was cl...

This study aimed to introduce a new method based on Hollow Fiber in order to determination of acetic anhydride (AA) in the air. Hydroxylamine hydrochloride and sodium hydroxide (1:1), were used as extraction solvent. This method was studied in the laboratory under various conditions. In order to make different concentrations of AA in the air, a standard glass chamber was used. The optimization ...

background: it has been demonstrated that insulin signaling pathway related genes have important roles in polycystic ovary syndrome (pcos) risk. the goal of present investigation was to assess the potential association between insr/nsii (rs2059806) and insr/pmli (rs1799817) snps and pcos. methods: 50 women with pcos and 47 normal controls entered the study. nsii and pmli snps in the insr gene w...