Ligation-mediated PCR was employed to quantify cyclobutane pyrimidine dimer (CPD) formation at nucleotide resolution along exon 2 of the adenine phosphoribosyltransferase (aprt) locus in Chinese hamster ovary (CHO) cells following irradiation with either UVA (340-400 nm), UVB (295-320 nm), UVC (254 nm) or simulated sunlight (SSL; lambda > 295 nm). The resulting DNA damage spectrum for each wave...

Benzo(a)pyrene (BaP) is a mutagenic carcinogen that is ubiquitous in our environment. To better understand the toxic effects of BaP and to explore the relationship between toxicity and toxicogenomics profiles, we assessed global mRNA and microRNA (miRNA) expression in Muta™Mouse. Adult male mice were exposed by oral gavage to 25, 50, and 75 mg/kg/day BaP for 28 days. Liver tissue was collected ...

Background: Recent descriptions of two new species genus Cutibacterium resulted in a further expansion the taxonomical tree genus, which include acnes. One species, modestum, was coined 2020 by presenter as fifth Cutibacterium, had been tentatively named “Propionibacterium humerusii”. Another porci, firstly isolated from porcine faeces Wylensek et al. 2020, being first possibly only non-human m...

Information on the genetic structure of fish is a useful means for optimizing identification stocks, stock enhancement, breeding programs, management sustainable yield and preservation diversity. The population African Catfish, Clarias gariepinus (Burchell, 1822) in cultured feral populations from Rivers Guzuguzu, Fada Magaga (Kano State) were investigated using RAPD (Random Amplified Polymorph...

Biostimulators with chemical protection are a challenge in sustainable agriculture to obtain high yield, healthy, and pesticide-free wheat. The aim of this four-year spring wheat field experiment was assess the effectivity using herbicide, mixed fungicides protection, humic biostimulator. following treatments were tested: biostimulator (S), sulfosulfuron (H), H + S, propiconazole cyproconazole/...

Samples of clinically normal oral tissue were obtained from patients undergoing surgery for intraoral squamous cell carcinoma. DNA was extracted from samples obtained from 20 tobacco smokers, four exsmokers, and nine nonsmokers and analyzed for the presence of aromatic DNA adducts using two distinct modifications of the '-I* postlabeling assay. ' V postlabeling following butano! extraction enha...

Sudden death, during or just after labour, has only too often been attributed to " obstetric shock." Out of this aetiological obscurity, in 1941 and 1942, Steiner and Lushbaugh extracted one recognizable cause of death, the finding in the maternal blood vessels of amniotic material. Recognition of other cases has followed rapidly (see review by Sluder and Lock, 1952), but thus far only three re...

Recently, a number of usage data representations have emerged that enable the representation of user activities across system and application boundaries. Based on these user activity data, systems can adapt to the users and provide personalized information. A lot of usage data representation formats are already successfully used in real world applications. However, dependent on the purpose, the...

A sensitive fluorometric assay using Hoechst 33258 and a modified alkaline elution procedure were used to quantitate DNA single-strand breaks following an in vivo drug treatment of mice bearing P-388/S and P-388/R cells. After an i.p. treatment of mice with 1 to 20 mg/kg Adriamycin (DOX), the following differences between sensitive and resistant P-388 cells were observed: (a) at 2 h following d...

Nitrogen mustard alkylating agents react with isolated DNA in a sequence selective manner, and the substituent attached to the drug reactive group can impose a distinct sequence preference. It is not clear however to what extent the observed DNA sequence preferences are preserved in intact cells. The highly reiterated sequence of human alpha DNA has been used to determine the sites of guanine-N...