نتایج جستجو برای: reporter gene
تعداد نتایج: 1151125 فیلتر نتایج به سال:
Bioluminescent and fluorescent influenza A viruses offer new opportunities to study influenza virus replication, tropism and pathogenesis. To date, several influenza A reporter viruses have been described. These strategies typically focused on a single reporter gene (either bioluminescent or fluorescent) in a single virus backbone. However, whilst bioluminescence is suited to in vivo imaging, f...
We describe a transient transfection protocol for cultured Leishmania major promastigotes, utilizing Escherichia coli genes encoding /?-galactosidase and B-glucuronidase inserted into an expression vector derived from the dihydrofolate reductasethymidylate synthase locus. Less than 0.1 pg of either reporter enzyme can be detected with a simple fluorimetric assay, and transfection of 10 pg of ei...
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