Laccases (EC 1.10.3.2) are multicopper oxidases that catalyze oxidation of various substituted phenolic compounds, aromatic amines and even certain inorganic compounds by using molecular oxygen as the electron acceptor. Their substrate versatility makes laccases highly interesting for various applications, including textile dye bleaching, pulp bleaching and bioremediation, where enzymatic catal...

introduction: the powdered activated carbon treatment (pact) has been proposed as an alternative in wastewater treatment and wastewater biomass protection against toxic substances. this study aims to evaluate pact performance in treating dyes, acid orange, and remazol brilliant reactive blue. methods: this empirical- applied research was carried out in pilot scale in which different dye removal...

When the living cells of Nitella are placed in a solution of brilliant cresyl blue containing NH(4)Cl, the rate of accumulation of the dye in the sap is found to be lower than when the cells are placed in a solution of dye containing no NH(4)Cl and this may occur without any increase in the pH value of the cell sap. This decrease is found to be primarily due to the presence of NH(3) in the sap ...

A DNA genomic library constructed from Bacillus stearothermophilus, a gram-positive, facultative thermophilic aerobe that secretes a thermostable beta-mannanase, was screened for mannan hydrolytic activity. Recombinant beta-mannanase activity was detected on the basis of the clearing of halos around Escherichia coli colonies grown on a dye-labelled substrate, Remazol brilliant blue-locust bean ...

By PHAEDON FESSAS F EW EXAMPLES of erythrocytic inclusions due to abnormalities of the hemoglobin molecule are known. The first concerns Hb H.”2 Although the precipitates of Hgb H, such as is observed in the usual test employing brilliant cresyl blue, are produced artificially, there is evidence that some are formed spontaneously in vivo.’4 The second example is that of Hb Zurich; the precipita...

In the present study, we investigated effects of different carbon sources (glucose, sucrose, and maltose) on laccase production from mycelium Ganoderma multistipitatum grown malt extract agar plates. The preliminary screening test was performed guaiacol plate, where a maroon brown zone formed after oxidation. A few pure mycelial discs species were transferred into submerged fermentation nutrien...

The rate of penetration of brilliant cresyl blue into the living cells of Nitella indicates that the dye enters only in the form of the undissociated molecule. At equilibrium the total concentration of the dye in the sap is proportional to the concentration of the free base in the outside solution.