A rapid and simple method for the simultaneous determination of twelve sweeteners and nine preservatives in various foods by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. The sweeteners and preservatives were extracted from solid samples with 80% and 50% methanol and from liquid samples with 80% methanol, followed by Oasis WAX cartridge cleanup. The LC separation was ...

The purpose of the present study was to determine the degree of synergism of sweet taste among ternary mixtures of 14 sweeteners. A trained panel evaluated ternary mixtures of 14 sweeteners varying in chemical structure and type. The ternary mixtures that were tested were limited to those in which the compounds comprising the mixture were synergistic in binary combinations, according to an earl...

Besides taste buds, many other tissues express receptors (TR), where they exert various, often ill-explored non-gustatory functions, e.g. in immunity and metabolism. These evolutionary ancient functions of human TRs can be optimally interrogated skin, whose keratinocytes selected TRs, its dominant appendage, the hair follicle (HF). However, role skin physiology is unknown. Therefore, we have as...

Stevia (Stevia rebuadiana Bertoni) is a perennial shrub grown for the extraction of sweet tasting, nonnutritive compounds called steviol glycosides (SGs) from leaves, which are up to 300 times sweeter than sugar. The most abundant SGs, stevioside and rebaudioside (Reb) A, have undesirable taste characteristics that limited their acceptance as sugar substitute. SGs Reb D M offer similar sweeteni...

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