a total of 200 moribund rainbow trout with clinical signs of a hyperacute haemorrhagic septicemia were collected from rainbow trout farms in fars, kohkiloyeh-boyer ahmad and charmohal-bakhtiari provinces in the south and southwest of iran during summer 2002 to winter 2008 for detection of lactococcus garvieae, the causative agent of lactococcosis. fish kidney samples were cultured aseptically o...

Non-targeted metagenomics offers the unprecedented possibility of simultaneously investigate the microbial profile and the genetic capabilities of a sample by a direct analysis of its entire DNA content. The assessment of the microbial taxonomic composition is frequently obtained by mapping reads to genomic databases that, although growing, are still limited and biased. Here we present riboFram...

Psyllids are plant sap-feeding insects that harbor prokaryotic endosymbionts in specialized cells within the body cavity. Four-kilobase DNA fragments containing 16S and 23S ribosomal DNA (rDNA) were amplified from the primary (P) endosymbiont of 32 species of psyllids representing three psyllid families and eight subfamilies. In addition, 0.54-kb fragments of the psyllid nuclear gene wingless w...

in recent years, environmental pollution by coal mining is a long-established human activity affecting all levels of life with various environmental impacts by generating heavy metals. the presence of heavy metals even in trace amount is toxic and detrimental to all living organisms. the coal mine area in bokaro is one of the “toxic hotspot” in india. bacteria have evolved uptake and efflux mec...

methyl tert-butyl ether (mtbe), a gasoline octane enhancer, was introduced as a substitute for lead tetraethyl over 30 years ago. widespread use of mtbe in gasoline, has introduced mtbe into the environment compartments, mostly into the under ground and surface water and water as a second most frequently detected contaminant. in this study, we have isolated pure cultures from bacterial consorti...

The identification of Mycobacteria in the species level has great medical importance. Biochemical tests are laborious and time-consuming, so new techniques could be used to identify the species. This research aimed to the comparison of biochemical and sequencing 16S rDNA gene methods to identify nontuberculous Mycobacteria in patients suspected to tuberculosis in Golestan province which is the ...