During World War I, when it became increasingly difficult for physicians in many European countries to have their defective X-ray tubes repaired or replaced, since most of these tubes were produced by German-based companies, Dutch physicians made an urgent request to Philips, asking the company if they could repair their X-ray tubes. Because Philips had the necessary expertise in glass and vacu...

We developed the double elution analysis technique, a modification of standard alkaline elution techniques, to assess cross-link formation and repair in the cycling and noncycling cell populations of multicellular spheroids. DNA cross-linking from cycling cells was measured using radioisotope incorporation; DNA cross-linking from the total cell popu lation was estimated using a fluorometric met...

We developed the double elution analysis technique, a modification of standard alkaline elution techniques, to assess cross-link formation and repair in the cycling and noncycling cell populations of multicellular spheroids. DNA cross-linking from cycling cells was measured using radioisotope incorporation; DNA cross-linking from the total cell population was estimated using a fluorometric meth...

Cross-linking agents that induce DNA interstrand cross-links (ICL) are widely used in anticancer chemotherapy. Yeast genetic studies show that nucleotide excision repair (NER), Rad6/Rad18-dependent postreplication repair, homologous recombination, and cell cycle checkpoint pathway are involved in ICL repair. To study the contribution of DNA damage response pathways in tolerance to cross-linking...

This paper describes the FSA Utilities toolbox: a collection of utilities to manipulate nite-state automata and nite-state transducers. Manipulations include determinization (both for nite-state accep-tors and nite-state transducers), minimization, composition, comple-mentation, intersection, Kleene closure, etc. Furthermore, various visu-alization tools are available to browse nite-state autom...

The interstrand crosslink (ICL) presents a challenge to both the cell and the scientist. From a clinical standpoint, these lesions are particularly intriguing: ICL-inducing agents are powerful tools in cancer chemotherapy, and spontaneous ICLs have recently been linked with accelerated aging phenotypes. Nevertheless, the ICL repair process has proven difficult to elucidate. Here we discuss rece...

Photoreactive psoralens can form interstrand crosslinks (ICLs) in double-stranded DNA. In eubacteria, the endonuclease UvrABC plays a key role in processing psoralen ICLs. Psoralen-modified triplex-forming oligonucleotides (TFOs) can be used to direct ICLs to specific genomic sites. Previous studies of pyrimidine-rich methoxypsoralen-modified TFOs indicated that the TFO inhibits cleavage by Uvr...

The aim of the present study was to determine whether specific molecular parameters may serve as predictors of treatment outcomes and toxicity of oxaliplatin (OXA)‑based chemotherapy, which is used as an adjuvant treatment in resected gastric cancer. All gastric cancer patients examined in the study received an OXA/5‑fluorouracil chemotherapeutic regimen. Genetic polymorphisms of certain platin...