نتایج جستجو برای: rat hepatocytes

تعداد نتایج: 290898  

Journal: :American journal of physiology. Endocrinology and metabolism 2012
Xabier Buqué Ainara Cano María E Miquilena-Colina Carmelo García-Monzón Begoña Ochoa Patricia Aspichueta

In myocytes and adipocytes, insulin increases fatty acid translocase (FAT)/CD36 translocation to the plasma membrane (PM), enhancing fatty acid (FA) uptake. Evidence links increased hepatic FAT/CD36 protein amount and gene expression with hyperinsulinemia in animal models and patients with fatty liver, but whether insulin regulates FAT/CD36 expression, amount, distribution, and function in hepa...

Journal: :Drug metabolism and disposition: the biological fate of chemicals 2006
Anne S Kienhuis Heleen M Wortelboer Jean-Christophe Hoflack Edwin J Moonen Jos C S Kleinjans Ben van Ommen Joost H M van Delft Rob H Stierum

Sandwich-cultured primary rat hepatocytes are often used as an in vitro model in toxicology and pharmacology. However, loss of liver-specific functions, in particular, the decline of cytochrome P450 (P450) enzyme activity, limits the value of this model for prediction of in vivo toxicity. In this study, we investigated whether a hepatic in vitro system with improved metabolic competence enhance...

Journal: :Drug metabolism and disposition: the biological fate of chemicals 2014
Patrik Lundquist Johan Lööf Urban Fagerholm Ingemo Sjögren Jenny Johansson Sveinn Briem Janet Hoogstraate Lovisa Afzelius Tommy B Andersson

Well-established techniques are available to predict in vivo hepatic uptake and metabolism from in vitro data, but predictive models for biliary clearance remain elusive. Several studies have verified the expression and activity of ATP-binding cassette (ABC) efflux transporters central to biliary clearance in freshly isolated rat hepatocytes, raising the possibility of predicting biliary cleara...

Journal: :Journal of lipid research 1994
G F Gibbons R Khurana A Odwell M C Seelaender

The human hepatoma cell line HepG2 in culture medium synthesized fatty acids de novo (144 +/- 9 nmol fatty acid/mg protein per 24 h) at a rate similar to that observed in freshly prepared rat hepatocytes (192 +/- 8 nmol/mg per 24 h) and in primary cultures of rat hepatocytes (165.4 +/- 29.3 nmol/mg per 24 h). In HepG2 cells, fatty acid synthesis was inhibited by extracellular oleate (0.75 mM), ...

Journal: :Indian journal of experimental biology 2010
V Sathya Srilakshmi P Vijayan P Vasanth Raj S A Dhanaraj H Raghu Chandrashekhar

Aim of the study was to investigate the methanol and aqueous extracts of heartwood of C. sappan for its hepatoprotective activity against CCl4 induced toxicity in freshly isolated rat hepatocytes and animals. Freshly isolated rat hepatocytes were exposed to CCl4 (1%) along with/without various concentrations of methanolic and aqueous extract of C. sappan (1000-800 microg/ml) and the levels of s...

Journal: :iranian journal of pharmaceutical sciences 0
ahmad salimi department of pharmacology and toxicology, faculty of pharmacy, shahid beheshti university of medical sciences, tehran, iran. department of pharmacology and toxicology, school of pharmacy, ardabil university of medical science, ardabil, iran. students research committee, school of pharmacy, shahid beheshti university of medical sciences, tehran, iran. melina ramandoost department of pharmacology and toxicology, faculty of pharmacy, shahid beheshti university of medical sciences, tehran, iran jalal pourahmad jaktaji department of pharmacology and toxicology, faculty of pharmacy, shahid beheshti university of medical sciences, tehran, iran

6- mercaptopurine (6-mp) is widely used in clinic as an immunosuppressive for treatment of acute lymphocytic leukemia, crohn's disease, and ulcerative colitis with documented unpredictable hepatotoxicity. the potential molecular cytotoxic mechanisms of 6-mp against isolated rat hepatocytes were searched in this study using “accelerated cytotoxicity mechanism screening (acms)” techniques. t...

Journal: :iranian journal of pharmaceutical sciences 0
mahdieh anoush department of pharmacology and toxicology, faculty of pharmacy, tabriz university of medical sciences, tabriz, iran department of pharmacology and toxicology, school of pharmacy, zanjan university of medical sciences, zanjan, iran mohammad ali eghbal department of pharmacology and toxicology, faculty of pharmacy, tabriz university of medical sciences, tabriz, iran. drug applied research center, tabriz university of medical sciences, tabriz, iran fatemeh fathiazad department of pharmacognosy, faculty of pharmacy, tabriz university of medical sciences, tabriz, iran hossein hamzeiy adepartment of pharmacology and toxicology, faculty of pharmacy, tabriz university of medical sciences, tabriz, iran. biotechnology research center, tabriz university of medical sciences, tabriz, iran nazli saeedi kouzehkonani drug applied research center, tabriz university of medical sciences, tabriz, iran

overdose of acetaminophen causes severe hepatic necrosis in humans and experimental animals. studies on its hepatotoxicity remain a very active area since some of current data are still uncertain. in this study, freshly isolated rat hepatocytes were used to determine the effects of garlic extract and its component, allicin on the acetaminophen-induced cell cytotoxicity and to compare with the e...

Journal: :The Biochemical journal 1993
M Decastel M A Doyennette-Moyne E Gouet M Aubery P Codogno

Zajdela hepatoma cells are poorly-adherent cells derived from an undifferentiated tumour and transplanted into rat. We compared the biosynthesis, structure and function of the fibronectin receptor in normal rat hepatocytes with that in Zajdela hepatoma cells. The rat hepatocyte fibronectin receptor has been isolated. It is composed of two subunits: alpha 5 (molecular mass 155 kDa) and beta 1 (m...

2002
H. J. Kempen

Lipoprotein production by freshly isolated rat hepatocytes in suspension was studied during short (1 -3 hr) incubation periods. The hepatocytes release very low density (d < 1.01 g/ml) lipoprotein (VLDL) particles, which as a group a) contain triacylglycerols, phospholipids, free cholesterol, and cholesteryl esters in molar proportions of 100:21:8:4; b) have a mean diameter 1.5-fold larger than...

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