The ability of stallion seminal plasma to modify phagocytosis of spermatozoa and Streptococcus zooepidemicus was examined. Phagocytosis was monitored indirectly as the H2O2 produced by peripheral blood leukocytes after addition of spermatozoa or bacteria. Hydrogen peroxide production after addition of ejaculated spermatozoa was greater (P less than 0.01) than after addition of epididymal sperm....

The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...

Disubstituted haloacid by-products of drinking water disinfection such as dibromoacetic acid and dichloroacetic acid have been shown to perturb spermatogenesis and fertility in adult male rats. In the present study we sought to establish whether equimolar exposure to bromochloroacetic acid (BCA), a prevalent by-product in finished drinking water, is also capable of disrupting these endpoints, a...

The effect of hypercholesterolaemia on testicular endocrine and exocrine function was evaluated. Furthermore, the influence of hypercholesterolaemia on sperm quality, quantity, and fertilizing potential was appreciated. Eight mature rabbits (group A) were fed chow containing 3% cholesterol for 12 weeks. An additional six rabbits (group B) were fed normal chow for the same period. At the end of ...

Background: The application of laparoscopic method for intrauterine insemination is limited in sheep industry by the high cost of equipment and low pregnancy rate of frozen-thawed spermatozoa. Both disadvantages could be decreased if chilled ram semen is transported to the farms and inseminated through cervix. Semen extender provides the requirements for sperm and affects its motility, viabilit...

BACKGROUND AND OBJECTIVES In this study, the harmful effects of formaldehyde (FA) on serum testosterone levels and epididymal sperm characteristics were investigated. In addition, possible protective effect of lavender oil was evaluated. MATERIALS AND METHODS For this purpose, 21 adult male Wistar-Albino rats were used. The rats of group I was used as control group. The rats of group II were ...

Sperm adsorbed with retrovirus particles were recovered from the epididymis of apparently normal male mice. Epididymal semen from all four mouse strains examined was positive for retrovirus (10(5) to 10(8) particles per microgram of protein) indicating that epididymal fluids and sperm may be important vehicles for murine retrovirus spread. Immunoblot analyses revealed that the banding patterns ...

Somatic cell nuclear transfer (SCNT) is a useful technique for creating pig strains that model human diseases. However, production of numerous cloned disease model pigs by SCNT for large-scale experiments is impractical due to its complexity and inefficiency. In the present study, we aimed to establish an efficient procedure for proliferating the diabetes model pig carrying the mutant human hep...

The mammalian spermatozoon has many cellular compartments, such as head and tail, permitting it to interact with the female reproductive tract and fertilize the egg. It acquires this fertilizing potential during transit through the epididymis, which secretes proteins that coat different sperm domains. Optimal levels of these proteins provide the spermatozoon with its ability to move to, bind to...

The current study aimed to examine the percentage changes of viability sperm and activity enzymes LDH, ALP, GOT / AST, GPT ALT in plasma Lacaune rams, before after cryopreservation. For this purpose, five rams were examined, two ejaculates obtained from each ram. Ejaculates are collected by method artificial vagina, during insemination campaign. All diluted with a 6AG extender frozen Cassou?s s...