In addition to the usual amino acids and vitamins, Lactobacilus delbrekii strain 730 (ATCC 9649) has been shown to require pyridoxamine phosphate (McNutt and Snell, 1950), thymidine (Kitay, et al., 1950), and an unsaturated fatty acid (e. g., oleic acid) (Kitay and Snell, 1950) for growth. In addition to these absolute requirements for growth, partial acid hydrolyzates of casein further stimula...

Cell-free extracts of rat kidney catalyze the conversion of kynurenine to kynurenic acid by means of a transamination reaction (2). Such extracts lose kynurenine transaminase activity rapidly when added to phosphate buffer solutions with pH below 7 unless ol-ketoglutarate is added (2). Further studies in this laboratory have shown that this inactivation is completely reversed by the addition of...

Pyridoxinamine 5'-phosphate oxidases (P(N/M)P oxidases) that bind flavin mononucleotide (FMN) and oxidize pyridoxine 5'-phosphate or pyridoxamine 5'-phosphate to form pyridoxal 5'-phosphate (PLP) are an important class of enzymes that play a central role in cell metabolism. Failure to generate an adequate supply of PLP is very detrimental to most organisms and is often clinically manifested as ...