Investigations of the effects of elevated ozone (O(3)) on the virus-plant system were conducted to inform virus pathogen management strategies better. One susceptible cultivar of tobacco (Nicotiana tabacum L. cv. Yongding) and a resistant cultivar (Nicotiana tabacum L. cv. Vam) to Potato virus Y petiole necrosis strain (PVY(N)) infection were grown in open-top chambers under ambient and elevate...

The cell-to-cell movement of the GUS-tagged potato virus X (PVX) coat protein (CP) movement-deficient mutant was restored by potyviral CPs of potato virus A (PVA) and potato virus Y (PVY) in Nicotiana benthamiana leaves in transient cobombardment experiments. Viral cell-to-cell movement of PVX CP mutant was complemented in Nicotiana tabacum cv. SR1 transgenic plants expressing PVY CP: PVX RNA a...

In spite of their widespread occurrence, only few host jumps by plant viruses have been evidenced and the molecular bases of even fewer have been determined. A combination of three independent approaches, 1) experimental evolution followed by reverse genetics analysis, 2) positive selection analysis, and 3) locus-by-locus analysis of molecular variance (AMOVA) allowed reconstructing the Potato ...

Abstract Mineral oils have been used for many decades to control the transmission of plant viruses and their ensuing epidemics. Although it is not clearly defined, mode action these has extensively studied, effects on feeding behaviour orientation vector or virus by its often put forward as possible explanations. This study examines effect mineral oil interaction between potato Y (PVY) vector, ...

*Part of the dissertation necessary for the M.Sc. degree in Plant Pathology of the first author, Universidade de Brasília (2002). CAPES fellowship Determination of virus diversity in the field is vital to support a sustainable breeding program for virus resistance of horticultural crops. The present study aimed to characterize four field potyvirus isolates found naturally infecting sweet pepper...

Potato tuber necrotic ringspot disease (PTNRD) is a tuber deformity associated with infection by the tuber necrotic strain of Potato virus Y (PVYNTN). PTNRD negatively impacts tuber quality and marketability, and poses a serious threat to seed and commercial potato production worldwide. PVYNTN symptoms differ in the cultivars Waneta and Pike: Waneta expresses severe PTNRD and foliar mosaic with...

Most routine testing for plant viruses is currently carried out using monoclonal and polyclonal antibodies. Traditional methods of antibody production however can be time consuming and require the use of expensive cell culture facilities. Recombinant antibody technology however is starting to make an impact in this area, enabling the selection of antibody fragments in a few weeks compared with ...

Potato spindle tuber viroid (PSTVd) is a harmful quarantine disease with wide geographic distribution. To date, experimentally proved resistance or tolerance of potato cultivars to PSTVd has not been reported. The aim this study was evaluate responses four strains 39 modern different origin. Four origin, the intermediate VP35, VP87, and two sever FP10-13 NicTr-3, deposited in GenBank, were used...

Abstract Over the past decades, two new strains of potato virus Y (PVY N-Wi (Wilga) and PVY NTN ) have been most widely distributed worldwide in crops ( Solanum tuberosum L.). Effective resistance genes comprehensive against all known are available some commercial cultivars. However, not cultivars challenged by to confirm effectiveness resistances available. This study was set identify resistan...