There are many thousand kinds of proteins with different properties and functions in a cell. To study a protein, one has to obtain the highly purified intact form of the protein. A major protein is not so difficult to be purified while a minor one may need many purification steps and high skills on the techniques. This chapter describes the principles underlying techniques for separation and pu...
O PuigF CasparyG RigautB RutzE BouveretE Bragado-NilssonM WilmB Séraphin
Identification of components present in biological complexes requires their purification to near homogeneity. Methods of purification vary from protein to protein, making it impossible to design a general purification strategy valid for all cases. We have developed the tandem affinity purification (TAP) method as a tool that allows rapid purification under native conditions of complexes, even w...
Charles L. Rutherford, Pawjai Khampang, Xiao Wen, Reyna Favis and Ian McCffery. Purification and Identification of a Protein that Binds to Regulatory Sites of the Glycogen Phosphorylase-2 Promoter of Dictyosteliym discoideum . International Dictyostelium Conference, October 13-18, 1996, Sendai, Japan