BACKGROUND Expression and purification of correctly folded proteins typically require screening of different parameters such as protein variants, solubility enhancing tags or expression hosts. Parallel vector series that cover all variations are available, but not without compromise. We have established a fast, efficient and absolutely background free cloning approach that can be applied to any...

background: bone morphogenetic protein 2 (bmp- 2) belongs to the tgf-β superfamily of proteins and plays an important role in the development of bone and cartilage. bmp- 2 is also associated with maintenance and repair of damaged bone. recombinant human bone morphogenetic protein 2 (rhbmp- 2) is now produced by genetic engineering techniques and used in treatment of thin bone fractures in the j...

The complete mouse mitochondrial genome (16.3 kb) was directly cloned into a Bacillus subtilis genome (BGM) vector. Two DNA segments of 2.06 and 2.14 kb that flank the internal 12 kb of the mitochondrial DNA (mtDNA) were subcloned into an Escherichia coli plasmid. Subsequent integration of the plasmid at the cloning locus of the BGM vector yielded a derivative specific for the targeted cloning ...

BACKGROUND The selection of bacterial recombinants that harbour a desired insert, has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the genes of interest. The conventional cloning techniques are reported to have problems such as screening high number of colonies, generation of false positives, setting up of cont...

cloning could be achieved by using this quick, alternative protocol. Not only does this Na3VO4-inhibition procedure save time, but also note that in this protocol the adjustment of vector-to-insert ratio in the ligation reaction is not necessary. To ligate with the CIAP/Na3VO4-treated vector (ca. 50 ng), we recommend using as much excess insert as is practical. Eliminating the quantitation step...

neospora caninum is an obligate intracellular parasitic protozoa and considered as causal agent of neosporosis which infect wide variety of hosts. ncgra7 is an immunodominant antigen recognized by sera from bovines, naturally infected by n. caninum, which is used as a powerful target for recombinant or dna vaccine preparation against neosporosis. there is no study about identifying the molecula...