ProSight PTM 2.0 (http://prosightptm2.scs.uiuc.edu) is the next generation of the ProSight PTM web-based system for the identification and characterization of proteins using top down tandem mass spectrometry. It introduces an entirely new data-driven interface, integrated Sequence Gazer for protein characterization, support for fixed modifications, terminal modifications and improved support fo...

The addition of posttranslational modifications (PTMs) to proteins is an influential mechanism to temporally control protein function and ultimately regulate entire cellular processes. Most PTMs are present at low stoichiometry and abundance, which limits their detection when analyzing whole cell lysates. PTM purification methods are thus required to comprehensively characterize the presence an...

BACKGROUND The impact of aortic prosthesis-patient mismatch (P-PtM) on long-term survival is unclear. METHODS AND RESULTS Between 1985 and 2000, 388 patients at Mayo Clinic in Rochester, Minn, underwent aortic valve replacement (AVR) with 19- or 21-mm St Jude Medical prostheses and had transthoracic echocardiography within 1 year after AVR. Mean age of patients was 62+/-13 years; 69% were fem...

Alterations in protein post-translational modification (PTM) and PTM cross-talk are increasingly being appreciated as driving mechanisms of human disease. The Protein Ontology (PRO) is a valuable resource to study the relation between PTM and disease because it represents individual proteins and protein complex subunits at the proteoform (e.g., isoform, PTM form, and sequence variant) level, wi...

Experiments were performed to determine whether or not acute exposure to elevated pressure would disrupt endothelium-dependent dilatation by increasing local angiotensin II (ANG II) signaling. Vasomotor responses of mouse-isolated carotid arteries were analyzed in a pressure myograph at a control transmural pressure (PTM) of 80 mmHg. Acetylcholine-induced dilatation was reduced by endothelial d...

Post-translational modifications (PTM) of proteins determine the activity, stability, specificity, transportability and lifespan of a protein. Some PTM are highly specific and regulated involving various enzymatic pathways, but there are other non-enzymatic PTM (nePTM), which occur stochastically, depend on the ternary structure of proteins and can be damaging. It is often observed that inactiv...

1. Total energy variation vs. twisting aryl rings in TPM and PTM molecules Fig. 1 The three aryl rings of the triphenylmethyl (TPM), (a) and perchloro-triarylmethyl (PTM), (b) TAM derivatives were systematically twisted from 20 to 90 degrees while relaxing the rest of the molecular structure by constrained optimizations. These calculations were done using the PBE0 hybrid functional 1 and the 6-...

Most companies that have developed their own technology have registered and patented the results. As a result, patent and technology management (PTM) has become important to companies needing to improve their market competitiveness. Using the PTM process, a company can develop a new product that is competitive in their market. First, we need to know the key technology (KT) a company uses to dev...

Autoantibodies have been associated with human pathologies for a long time, particularly with autoimmune diseases (AIDs). Rheumatoid factor (RF) is known since the late 1930s to be associated with rheumatoid arthritis (RA). The discovery of anticitrullinated protein antibodies in the last century has changed this and other posttranslational modifications (PTM) relevant to RA have since been des...

1. We investigated the association between field body temperatures (Tb), field air temperatures (Ta), and their differences (D) with measurements of foraging activity (percentage of time moving (PTM), number of movements per minute (MPM) and proportion of prey attacked while moving (PAM)) for 25 species of lacertid lizards. 2. Lizards active at relatively high field body temperatures tended to ...