The sequential resonance assignment of the 1H and 15N NMR spectra of the DNA binding protein Ner from phage Mu is presented. This is carried out by using a combination of 1H-1H and 1H-15N two-dimensional experiments. The availability of completely labeled 15N protein enabled us to record a variety of relayed heteronuclear multiple quantum coherence experiments, thereby enabling the correlation ...

This work summarizes the electrochemical response of flavanone carbon composite electrodes in comparison with Nafion®-coated electrodes, for use as voltammetric pH sensors both buffered and low-buffered media. Square wave measurements suggest peak potential achieved from polymerization after electron-proton oxidation responds accuracy to solutions coated non-coated a shift 55.1 mV 54.6 per unit...

Both neutralophilic Bacillus subtilis and alkaliphilic Bacillus firmus OF4 depend upon electrogenic Na+/H+ antiporters, which are energized by the gradients established by respiration-coupled proton extrusion, to achieve Na(+)-resistance and pH homeostasis when the external pH is very alkaline. The interplay of proton and sodium cycles is discussed. In B. subtilis, pH homeostasis, up to pH9, ca...

The hangman motif provides mechanistic insights into the role of pendant proton relays in governing proton-coupled electron transfer (PCET) involved in the hydrogen evolution reaction (HER). We now show improved HER activity of Ni compared with Co hangman porphyrins. Cyclic voltammogram data and simulations, together with computational studies using density functional theory, implicate a shift ...

Ribozymes of hepatitis delta virus have been proposed to use an active-site cytosine as an acid-base catalyst in the self-cleavage reaction. In this study, we have examined the role of cytosine in more detail with the antigenomic ribozyme. Evidence that proton transfer in the rate-determining step involved cytosine 76 (C76) was obtained from examining cleavage activity of the wild-type and imid...

Rimantadine hydrochloride (α-methyl-1-adamantane-methalamine hydrochloride) is a chiral compound which exerts antiviral activity against the influenza A virus by inhibiting proton conductance of the M2 ion channel. In complex with M2, rimantadine has always been characterized as a racemic mixture. Here, we report the novel enantioselective synthesis of deuterium-labeled (R)- and (S)-rimantadine...

Proton decoupling and nuclear Overhauser effect (NOE) enhancement significantly improve the signal-to-noise ratio and enhance resolution of metabolites in in vivo 31P MRS. We obtained proton-decoupled, NOE-enhanced, phospholipid-saturated 31P spectra localized to defined regions within the normal liver using three-dimensional chemical shift imaging. Proton-decoupling resulted in the resolution ...

To compensate the large tune shift and tune spread generated by the head-on beam-beam interactions in polarized proton operation in the Relativistic Heavy Ion Collider (RHIC), a low energy electron beam with proper Gaussian transverse profiles was proposed to collide head-on with the proton beam. In this article, using a modified version of SixTrack [1], we investigate stability of the single p...

High-resolution Fourier transform NMR at 15.08 MHz was used to observe the proton-decoupled natural-abundance (13)C spectra of aqueous solutions of cobinamide dicyanide (0.067 M), cyanocobalamin (0.024 M), dicyanocobalamin (0.14 M), and coenzyme B(12) (0.038 M). Assignments were made with the aid of chemical shift comparisons, off-resonance single-frequency proton decoupling, partially-relaxed ...