As part of an ongoing characterization of the intrinsic chemical properties of peptides, thermal hydrogen-deuterium exchange has been studied for a series of fast-atom-bombardment-generated protonated alkyldipeptides and related model compounds in the reaction with D2O, CH3OD, and ND3 in a Fourier transform ion cyclotron resonance mass spectrometer. Despite the very large basicity difference be...

A method for detecting structure in marginally stable forms of a protein is described. The principle is to measure amide proton exchange rates in the absence and presence of varying concentrations of a denaturant. Unfolding of structure by the denaturant is reflected by an acceleration of amide proton exchange rates, after correction for the effects of the denaturant on the intrinsic rate of ex...

Proton exchange membrane fuel cells have been causing attention because of theirs many advantages. In order to maintain good working condition good controller is required. In this study, the mathematical model for proton exchange membrane fuel cell is developed. Then combining the characteristics of fuzzy control and sliding mode control fuzzy sliding mode controller is designed. The controller...

We calculate in heavy baryon chiral perturbation theory the local NN-potentials generated by the two-pion exchange diagrams at two-loop order. We give explicit expressions for the mass-spectra (or imaginary parts) of the corresponding isoscalar and isovector central, spin-spin and tensor NN-amplitudes. We find from two-loop two-pion exchange a sizeable isoscalar central repulsion which amounts ...

Twelve binary and eight ternary H-bonded systems between uracil and water=methanol were investigated at the B3LYP and MP2 theoretical levels using 6 31þG(d) basis functions. The binary and ternary systems that contain the hydroxo-uracil tautomer H-bonded with water and methanol were found to be the most stable complexes. The calculated energy barriers of the intermolecular proton exchange showe...

The well-resolved base and sugar proton resonances in the high resolution proton nuclear magnetic resonance (NMR) spectra of poly(dA-dT) can be monitored during the helix-coil transition. The observable resonances shift upfield on helix formation and the temperature-dependent chemical shifts exhibit a melting temperature t 1/2 = 69.9 +/- 0.3 degrees for 18.8 mM (with respect to phosphorus) poly...

The present paper explores the impact of an on-board hydrogen harvesting system (fuel reformer) on the overall efficiency of a fuel-cell powered vehicle. Various methods of hydrogen production for automotive applications have been discussed first. As the hydrogen production is one of the major challenges for application of proton exchange membrane (PEM) fuel cells, especially in vehicular indus...

Aspartic acid (Asp)-containing peptides with the fixed charge derivative tris(2,4,6trimethoxyphenyl) phosphonium (tTMP-P ) were explored computationally and experimentally by hydrogen/deuterium (H/D) exchange and by fragmentation studies to probe the phenomenon of selective cleavage C-terminal to Asp in the absence of a “mobile” proton. Ab initio modeling of the tTMP-P electrostatic potential s...

Regioselective labelling of arene rings via electrophilic exchange is often dictated by the electronic environment caused by substituents present on the aromatic system. Previously, we observed the presence of a t-butyl group, either covalently bond or added as an external reagent, could impart deuterium exchange to the unactivated, C1-position of estrone. Here, we provide nuclear magnetic reso...