نتایج جستجو برای: proteolytic extract
تعداد نتایج: 198603 فیلتر نتایج به سال:
Here, we report the discovery of first plant-derived and noncanonical epidermal growth factor receptor (EGFR) agonist, 36-residue bleogen pB1 from Pereskia bleo Cactaceae family. We show that is a low-affinity EGFR agonist using suite chemical, biochemical, cellular, animal experiments which include incisor eruption wound-healing mouse models. A focused positional scanning library Ala- d-amino ...
alkaline crude enzymes from the viscera of the tunisian barbel (barbus callensis) were extracted and characterized. proteolytic crude extract from barbel viscera was active and stable in alkaline solution. the optimum ph and temperature were 11.0 and 55 °c, respectively, using casein as a substrate. the crude alkaline protease was extremely stable in the ph range of 5.0-12.0. zymography activit...
The migration of different cell types, such as leucocytes and tumour cells, involves cellular strategies to overcome the physical resistance of three-dimensional tissue networks, including proteolytic degradation of extracellular matrix (ECM) components. High-resolution live-cell imaging techniques have recently provided structural and biochemical insight into the differential use of matrix-deg...
We used polyclonal serum raised against mature trichocyst matrix proteins to detect their unprocessed precursors, a group of proteins (45-55 kDa) present in the whole-cell extract. These precursor proteins were partially purified from the soluble fraction of wild-type cells by ammonium sulfate precipitation and anion-exchange chromatography. Using monoclonal antibodies against each of four fami...
The traditional Krebs procedure (1) for preparing yeast glyceraldehyde 3-phosphate dehydrogenasc has many advantages, but it also has some serious disadvantages. The procedure requires long periods of waiting during the first few steps of making the crude extract, and is llot reproducible in the quality of the enzyme obtained. Lazarus et al. (2) have shown that proteolytic enzymes survive the t...
Renal intra-arterial administration of rat atrial extracts elicits a concentration dependent renal vasodilation (independent of prostaglandin or dopamine release) in anesthetized rats. The atrial extracts do not alter skeletal musculature (hindlimb) vascular resistance or systemic arterial blood pressure. The high molecular weight peptide fraction of atrial extracts obtained by gel filtration, ...
Most of the species belonging to Asclepiadaceae family usually secrete an endogenous milk-like fluid in a network of laticifer cells in which sub-cellular organelles intensively synthesize proteins and secondary metabolites. A new papain-like endopeptidase (asclepain c-II) has been isolated and characterized from the latex extracted from petioles of Asclepias curassavica L. (Asclepiadaceae). As...
Reactive oxygen species (ROS) produced from cigarette smoke cause oxidative lung damage including protein denaturation, lipid peroxidation, and DNA damage. Glutathione peroxidase-1 (gpx-1) is a detoxifying enzyme that may protect lungs from such damage. The aim of this study was to determine whether gpx-1 protects the lung against oxidative stress-induced lung inflammation in vivo. Male wild-ty...
A considerable number of intracellular animal proteases have been described (1,2). Many of the enzymes studied exhibit pH optima in the acid range, whereas some show optimal activity at or near neutrality. Relatively few of the cathepsins described to date show maximal activity in the alkaline pH range. One such protease, found in aqueous extracts of whole rat lung, exhibits a pH optimum at 8.4...
Characterization of partially purified milk‐clotting enzyme from sunflower (Helianthus annuus) seeds
This study was aimed to extract milk-clotting enzyme from sunflower seeds and to determine its potentiality for manufacturing white soft cheese from cows and goats milk. The seeds were blended and extracted using two types of buffers and milk-clotting and proteolytic activities were evaluated. The enzyme was partially purified using ammonium sulfate fractionation techniques. Results indicated t...
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