neospora caninum is an obligate intracellular parasitic protozoa and considered as causal agent of neosporosis which infect wide variety of hosts. ncgra7 is an immunodominant antigen recognized by sera from bovines, naturally infected by n. caninum, which is used as a powerful target for recombinant or dna vaccine preparation against neosporosis. there is no study about identifying the molecula...

background the x-linked cyclin-dependent kinase like 5 (cdkl5/stk9) gene has been shown to be responsible for a severe encephalopathy condition characterized by early onset of epilepsy and severe developmental delay. cdkl5 mutations have been shown to be more frequent among female patients. results here we report a 6- month male patient, second child of a healthy non consanguineous in the irani...

Despite significant advances in the identification of known proteins, the analysis of unknown proteins by tandem mass spectrometry (MS/MS) still remains a challenging open problem. Although Klaus Biemann recognized the potential of tandem mass spectrometry (MS/MS) for sequencing of unknown proteins in the 1980s, low-throughput Edman degradation followed by cloning still remains the main method ...

objective(s): streptococcus pyogenes produces extracellular hyaluronidase enzyme. this enzyme is directly associated with the spread of the organism during infection. the objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. materials and methods: the enzymatic region of hyaluronidase gene was detecte...

objective(s) leishmania can lead to a broad spectrum of diseases, collectively known as leishmaniasis. the a2 gene/ protein family could be one of the most eligible candidate factors of virulence in visceral leishmaniasis (vl). the previous results confirmed that in leishmania infantum, several a2 proteins are abundantly expressed by the amastigote, but not the promastigote stage. as there are ...

The recent advantages of the molecular biologic methods have a deep infuence on routine dermatopathology, especially in case melanoma. fingerprint melanoma has resulted tissue validation potential prognostic and predictive biomarkers. In addition to DNA-, RNA-based techniques, protein sequencing contributes OMIC strategy, affecting dermatopathology labs. paper summarizes biological aspects diag...