Background: Protein-protein interactions do not provide any direct information re‌garding the domains within the proteins that mediate the interactions. The majority of proteins are multi domain proteins and the interaction between them is often defined by the pairs of their domains. Most of the former studies focus only on interacting do‌main pairs. However they do not consider the in...

We have previously shown that TFII-I enhances transcriptional activation of the c-fos promoter through interactions with upstream elements in a signal-dependent manner. Here we demonstrate that activated Ras and RhoA synergize with TFII-I for c-fos promoter activation, whereas dominant-negative Ras and RhoA inhibit these effects of TFII-I. The Mek1 inhibitor, PD98059 abrogates the enhancement o...

Knowledge of all molecular interactions that potentially take place in the cell is a key for a detailed understanding of cellular processes. Currently available interaction data, such as protein-protein interaction maps, are known to contain false positives that inevitably diminish the accuracy of network-based inferences. Interaction confidence scoring is thus a crucial intermediate step after...

lymphomas are solid tumors of immune system and non-hodgkin lymphomas (nhl) is the most prevalent lymphomas; with wide ranges of histological and clinical features, it is so difficult to identify them. herein, various bioinformatics tools (such as gene differential expressions, epigenetics and protein analysis) employed to find new treatment approach for nhl based on gene expression variation b...

Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map prote...

MAP kinase phosphatase (MKP)-3 is a cytoplasmic dual specificity protein phosphatase that specifically binds to and inactivates the ERK1/2 MAP kinases in mammalian cells. However, the molecular basis of the cytoplasmic localization of MKP-3 or its physiological significance is unknown. We have used MKP-3-green fluorescent protein fusions in conjunction with leptomycin B to show that the cytopla...

Human immunodeficiency virus type 1 (HIV-1) exploits a diverse array of host cell functions in order to replicate. This is mediated through a network of virus-host interactions. A variety of recent studies have catalogued this information. In particular the HIV-1, Human Protein Interaction Database (HHPID) has provided a unique depth of protein interaction detail. However, as a map of HIV-1 inf...

Background and objectives: Positive role of saffron in human health promotion has been investigated in widespread researches. Anticancer property, neuroprotection, protection of cardiovascular system and several positive properties are reported for saffron customers. The aim of this study was assessment of saffron weakness against light damage in rat retina. Methods:</s...

The advent of high-throughput experiments to measure protein–protein interactions has created a flood of proteomic information parallel to the influx of data caused by the advent of next-generation sequencing technologies. The creation of whole organism protein interaction maps has opened new avenues of predicting genetic disease. Advances in network science now allow the association of genes w...