1. When isolated kidneys from fed rats were perfused with glutamine the rate of ammonia release at pH7.4 (110-360mumol/h per g dry wt.) was one to two times that of glutamine removal. Glucose formation from 5mm-glutamine was 16mumol/h per g. If kidneys were perfused with glutamine at pH7.1 (10-13mm-sodium bicarbonate) there was no increase in glutamine removal or in the formation of ammonia or ...

Introduction Glutamine is the source for most of the nitrogen in biosynthetic pathways because of its connection to carbohydrate metabolism via cr-oxoglutarate and glutamate. The enzymes that transfer nitrogen from the amide of glutamine to acceptor substrates in biosynthetic pathways are known as glutamine amidotransferases. Glutamine amidotransferases are a large and well studied family of en...

INTRODUCTION Low plasma glutamine levels are associated with worse clinical outcome. Intravenous glutamine infusion dose- dependently increases plasma glutamine levels, thereby correcting hypoglutaminemia. Glutamine may be transformed to glutamate which might limit its application at a higher dose in patients with severe traumatic brain injury (TBI). To date, the optimal glutamine dose required...

Endogenous production of glutamine may become insufficient during critical illness. The shortage of glutamine is reflected as a decrease in plasma concentration, which is a prognostic factor for poor outcome in sepsis. Because glutamine is a precursor for nucleotide synthesis, rapidly dividing cells are most likely to suffer from a shortage. Therefore, exogenous glutamine supplementation is nec...

ammonia formation as well as ammonia formation from glutamine in kidney slices (3, 7, 10). Since glutamine stimulates oxygen uptake by the slices, it has been suggested that this effect is due to the anaerobic inhibition of alpha amino oxidase. Anoxia also inhibits endogenous ammonia forma tion in brain, but glutamine is here excluded as the source of ammonia (1@, 14). Ehrlich ascites tumor cel...

From the perspective of nutritional science, glutamine, like arginine, is not an essential amino acid. Nevertheless, glutamine is the most abundant amino acid in the body. Glutamine plays major roles in accelerating muscle metabolism, in preserving muscle, in promoting wound healing and in cell proliferation. It also serves as the nutritional source for gastrointestinal cells and as the major e...

The liver shows net glutamine uptake after a protein-containing meal, during uncontrolled diabetes, sepsis and short-term starvation, but changes to net release during long-term starvation and metabolic acidosis. Some studies report a small net release of glutamate by the liver. The differential expression of glutamine synthetase (perivenous) and glutaminase (periportal) within the liver indica...

1. Suspensions of isolated chick jejunal columnar absorptive (brush-border) cells respired on endogenous substrates at a rate 40% higher than that shown by rat brush-border cells. 2. Added d-glucose (5 or 10mm), l-glutamine (2.5mm) and l-glutamate (2.5mm) were the only individual substrates which stimulated respiration by chick cells; l-aspartate (2.5 or 6.7mm), glutamate (6.7mm), glutamine (6....

The metabolism of (5-15N)glutamine and (2-15N) glutamine has been studied by isolated hepatocytes obtained from either control, chronically acidotic, or alkalotic rats. The main goal was to elucidate the mechanism(s) by which altered acid-base state affects hepatic ureagenesis from glutamine. Isolated hepatocytes were incubated in Krebs buffer (pH 7.4) supplemented with 0.1 mM ornithine plus ei...