Using 20 strains of Pseudomonas aeruginosa isolated from patients, production of protease, elastase, and collagenase was determined by shaking culture in either complex or semisynthetic medium. No collagenase was produced by any strain of P. aeruginosa. According to their production of protease and elastase in different media, the P. aeruginosa strains were divided into three groups: the first ...

Growth of Bacillus licheniformis in a chemically defined medium containing glucose and ammonium chloride yielded a doubling time of 1.00 h. Examination of the culture during exponential growth revealed a lack of heat-resistant spores together with a complete absence of detectable concentrations of bacitracin or extracellular serine protease. Replacement of glucose as the sole carbon source by g...

Previous studies had shown that sub-inhibitory concentrations of silver sulphadiazine (AgSD) stimulated the production of Toxic Shock Syndrome Toxin-1 in certain strains (responder strains) of Staphylococcus aureus and that protease production was also affected. No changes were detected in other strains (non-responders). Extracellular proteins from eleven responder and non-responder strains gro...

Previous studies had shown that sub-inhibitory concentrations of silver sulphadiazine (AgSD) stimulated the production of Toxic Shock Syndrome Toxin-1 in certain strains (responder strains) of Staphylococcus aureus and that protease production was also affected. No changes were detected in other strains (non-responders). Extracellular proteins from eleven responder and non-responder strains gro...

The production of halophilic thermostable protease by Haloferax lucentensis VKMM 007 was optimized using a statistical approach. In accordance with factorial design, soluble starch, gelatin, KCl and MgSO4 were selected among 27 variables tested. Next, a second-order quadratic model was estimated and optimal medium concentrations were determined based on quadratic regression equation generated b...

Protease executes a large variety of functions and it has many biotechnological applications. It represents as one of the third largest group of industrial enzyme and finds its applications in detergents, leather industry, pharmaceutical industry etc. Among the different vegetable waste products, peel of Manihot esculenta (Tapioca/ cassava) was used as substrates for the production of protease ...

The aim of this study was to use agro-industrial residues for the production of a halotolerant keratinolyticprotease by Actinobacterium sp. in solid-state fermentation. Among various agro-industrial residues that were evaluated, apple pomace supported maximum protease production (8400 U/g material). The optimum conditions required for enzyme production were a fermentation period of 72 h, 10% (w...

In this study, we have shown the microbial alkaline protease production by using isolated Bacillus subtilis. Bacillus subtilis which produces an extracellular alkaline protease was isolated from meet surface purchased from local Egyptian markets. Maximum enzyme activity was achieved when the bacterium was grown on corn steep liquor (2.0 %, w/v) instead of soybean meal (2 %) followed by casein h...

Protease production under solid state fermentation (SSF) was investigated using isolated Aspergillus flavus. Different agroindustrial waste products were evaluated to check the possibility of potential utilization of substrates in SSF for protease production by Aspergillus flavus using wheat bran as a substrate. The results showed that the optimum conditions for maximum protease production were...