The crystal structure of cytochrome c nitrite reductase (NrfA) from D. desulfuricans ATCC 27774 was determined to 2.3 Å resolution [1]. In solution the protein was purified as a hetero-oligomer of two different subunits, the catalytic subunit NrfA (62 kDa) and a smaller subunit (19 kDa) that corresponds to the membrane-bound subunit NrfH [2]. NrfA possesses five c-type hemes per monomer and cat...

The complete three-dimensional sensory module structures of the Pr ground state of Synechocystis 6803 Cph1 and the unusual Pfr ground state of the bacteriophytochrome PaBphP (PDB codes 2VEA and 3C2W respectively) have now been solved, revealing an asymmetrical dumbbell form made up of a PAS (Period/ARNT/Singleminded)-GAF (cGMP phosphodiesterase/adenylate cyclase/FhlA) bidomain carrying the chro...

Emergence of tuberculosis as a global health threat has necessitated an urgent search for new antitubercular drugs entailing determination of 3-dimensional structures of a large number of mycobacterial proteins for structure-based drug design. The essential requirement of ferritins/bacterioferritins (proteins involved in iron storage and homeostasis) for the survival of several prokaryotic path...

Sperm whale myoglobin, an oxygen storage hemoprotein, was successfully reconstituted with the iron porphycene having two propionates, 2,7-diethyl-3,6,12,17-tetramethyl-13,16-bis(carboxyethyl)porphycenatoiron. The physicochemical properties and ligand bindings of the reconstituted myoglobin were investigated. The ferric reconstituted myoglobin shows the remarkable stability against acid denatura...

The negative effect of pH on rumen microbial fermentation has been associated with the total amount of time that pH is below a certain threshold. However, not only the time, but also the magnitude of the pH reduction, is important. Eight 1,325-mL dual-flow continuous culture fermenters were used in 2 replicated periods to determine the effect of the magnitude of pH reduction (5.6 vs. 5.1) durin...

Large amounts of homopolymers containing -hydroxybutyrate (PHB) and copolymers containing -hydroxyvalerate (P[HB-co-HV]) are produced by Azotobacter chroococcum strain H23 when growing in culture media amended with alpechı́n (wastewater from olive oil mills) as the sole carbon source. Copolymer was formed when valerate (pentanoate) was added as a precursor to the alpechı́n medium, but it was not ...

The uptake of propionate by the isolated perfused rat liver is not influenced by oleate or glucagon. However, glucagon does stimulate glucose production in the presence of 10 flwl propionate and increases the incorporation of isotope from [14C]propionate into glucose. These observations have been interpreted to indicate a sparing of the metabolism of propionate in the citric acid cycle by endog...

Patterns of (sup14)CO(inf2) incorporation into molecular components of the thermophilic cyanobacterial mat communities inhabiting hot springs located in Yellowstone National Park and Synechococcus sp. strain C1 were investigated. Exponentially growing Synechococcus sp. strain C1 partitioned the majority of incorporated (sup14)CO(inf2) into protein, low-molecular-weight metabolites, and lipid fr...

Current pharmacokinetic (PK) bioequivalence guidelines do not account for batch-to-batch variability in study design or analysis. Here we evaluate the magnitude of batch-to-batch PK variability for Advair Diskus 100/50. Single doses of fluticasone propionate and salmeterol combinations were administered by oral inhalation to healthy subjects in a randomized clinical crossover study comparing th...

1, Curzon Road, New Delhi Testosterone propionate, the synthetic male sex hormone, is a potent therapeutic agent in the treatment of certain male and female disorders'. It was first isolated from bulls' testes and later synthetically from cholesterol. It has been used clinically with marked success by many workers. In spite, however, of the fact that much has been written about it in recent yea...