Substrate oxidation and amino acid transport were examined in the presence of sulfhydryl reagents with membrane preparations from Mycobacferium phlei. Oxidation of exogenously added @-NADH, succinate, ascorbate N, N, N’, IV’-tetramethyl-p-phenylenediamine, or ascorbate phenazinc methosulfate was inhibited by organic mercurials. The organic mercurials inhibited succinate oxidation between cytoch...

Proline accumulation is a well-known response to water deficits in leaves. The primary cause of accumulation is proline synthesis. Delta(1)-Pyrroline-5-carboxylate reductase (PCR) catalyzes the final reaction of proline synthesis. To determine the subcellular location of PCR, protoplasts were made from leaves of Pisum sativum L., lysed, and fractionated by differential and Percoll density gradi...

Ling, Chung-Mei (Illinois Institute of Technology, Chicago), and L. R. Hedrick. Proline oxidases in Hansenula subpelliculosa. J. Bacteriol. 87:1462-1470. 1964-Cells of Hansenula subpelliculosa can use l-proline as a carbon and a nitrogen source after a 6- to 8-hr induction period. However, they cannot use l-glutamate as both nitrogen and carbon sources unless the induction period is of several ...

Legume root nodule nitrogen-fixing activity is severely affected by osmotic stress. Proline accumulation has been shown to induce tolerance to salt stress, and transgenic plants over-expressing Delta(1)-pyrroline-5-carboxylate synthetase (P5CS), which accumulates high levels of proline, display enhanced osmotolerance. Here, we transformed the model legume Medicago truncatula with the P5CS gene ...

Active transport of proline was observed in both electron transport particles and cell membrane ghosts from Mycobacterium phlei. The transport of proline was dependent upon substrate oxidation and the presence of Li+ or Na+, but not upon the formation of a high energy phosphorylated intermediate. The requirement for Na+ was different from Na+ coupled transport systems which have been described;...