Electrophoresis of haemolysates on a starch block, which was first described by Kunkel and Wallenius (1955), provides an excellent method for separating and quantitating the various normal and abnormal haemoglobins. Since relatively large amounts of haemoglobin solutions can be applied it is a useful preparative method for isolating a particular haemoglobin for further analysis. One of the impo...

The C'1a-fixing properties of purified rabbit IgM anti-benzenearsonate antibody were determined. When tested with sheep erythrocytes to which hapten had been coupled by diazo linkage, the number of C'1a molecules fixed was 21% of the number of IgM antibody molecules bound to the erythrocyte surface. This was not due to loss of C'1a-fixing capacity during the purification procedure. Preparative ...

We have purified and characterized a DNA-dependent RNA polymerase from the blue-green alga Fremyella diplosiphon. This enzyme, purified by gel filtration, DEAE-cellulose chromatography, and glycerol gradient centrifugation, is comprised of five polypeptide subunits. Their masses are 161,000, 134,000, 91,000, 72,000, and 41,000 daltons. Preparative electrophoresis of the purified enzyme on nonde...

Lysine-epsilon-C(14)-labeled plasma proteins produced by the normal rat and the isolated perfused rat liver have been fractionated by preparative zone electrophoresis. The isolated perfused liver incorporates lysine-epsilon-C(14) into the plasma albumin, alpha globulin, and beta globulin (including fibrinogen) fractions. No significant C(14) incorporation into the trichloracetic acid-precipitab...

Intermediate filaments (IF) were reconstituted in vitro from bovine neurofilament triplet polypeptides. Neural IF, solubilized in either low salt or 8 M urea solution, assembled into IF when returned to near-physiological solution conditions. The 68,000-dalton component of the triplet, purified to homogeneity by preparative NaDodSO4 electrophoresis, was renatured and reassembled into short (app...

Twelve of the proteins from the 30S ribosome of Bacillus stearothermophilus were isolated by preparative disc electrophoresis. Amino acid analyses of these proteins showed them to be different from each other. The gross amino acid composition of 30S ribosomal protein from B. stearothermophilus and Escherichia coli are virtually identical. A number of the proteins of B. stearothermophilus had el...

Mitogen-stimulated mononuclear blood cells produce differentiation inducing factors (DIFs) for the promyelocytic cell line HL-60. We report that DIF is produced constitutively by a malignant T lymphocyte line HUT-102. DIF was purified 7.000-fold from HUT-i 02 conditioned media by utilizing ion-exchange chromatography with DEAE-Sepharose, gel chromatography, Blue-Sepharose chromatography. and pr...

N. Leigh Anderson Molecular Anatomy Program Division of Biological and Medical Research Argonne National Laboratory Argonne, Illinois 60439 Received June 6,1979 SUMMARY: Using the technique of thermal denaturation in a temperature gradient followed by two-dimensional gel electrophoresis of the fractions, the thermostabilities of the cytoplasmic actins have been investigated. Addition of 5 mM Mg...

A novel o-phthalaldehyde-reactive compound was found in the h.p.l.c. chromatogram of Aplysia kurodai extract. This compound was isolated by ion-exchange chromatography and preparative high-voltage paper electrophoresis. It was shown by optical-rotatory-dispersion spectrum and optical-resolution h.p.l.c. analysis that this compound consisted of equimolar amounts of D-aspartic acid and glycine. T...

The positions of the disulfide bonds in Cerebratulus lacteus toxin B-IV were investigated by hydrolysis of the unreduced protein with a variety of proteases. The resulting peptides were purified by gel filtration, ion exchange chromatography, and preparative paper electrophoresis and chromatography. Determination of the amino acid compositions of the cystine-containing peptides purified demonst...