Preconditioning by volatile anaesthetics is blocked by hyperglycaemia. The regulation of mitogen-activated protein kinases during this effect has yet not been investigated. For infarct size measurements, anaesthetized rats were subjected to 25 min coronary artery occlusion followed by 120 min reperfusion. Control animals were not further treated. One group was preconditioned by two 5-min period...

TNF alpha administration mimics ischemic preconditioning and neutralizing antibodies to TNF alpha and IL-1 beta abolish exercise-induced preconditioning. However, the pharmacology of TNF alpha's cardioprotective effects and associated downstream signaling events has not been delineated. We evaluated the temporal and dose specific requirements of TNF alpha to function as a preconditioning mimeti...

Both the phosphatidylinositol 3-kinase (PI3K)/Akt pathway and heme oxygenase-1 (HO-1) create a survival signal against oxidative stress-induced injuries. Although we have demonstrated that hydrogen peroxide (H2O2) preconditioning confers adaptive cytoprotection against oxidative stress-induced injury in PC12 cells, it remains unknown whether these defense systems are involved in the protective ...

In December 2011, the 2 nd Translational Preconditioning Meeting was held at the University of Miami Miller School of Medicine. The motivation for this meeting arose from the success of the first meeting organized by Dr Guohua Xi and Dr Richard Keep at the University of Michigan, which took place in Ann Arbor in 2009. The main goal of the Miami meeting was to discuss and identify effective stra...

Increasing evidence indicates that argon has organoprotective properties. So far, the underlying mechanisms remain poorly understood. Therefore, we investigated the effect of argon preconditioning in cardiomyocytes within the first and second window of preconditioning. Primary isolated cardiomyocytes from neonatal rats were subjected to 50% argon for 1 h, and subsequently exposed to a sublethal...

Ischemic preconditioning refers to the endogenous mechanism of protection against a sustained ischemic insult following an initial, brief ischemic stimulus. Ischemia-reperfusion injury of the liver is a major cause of morbidity and mortality in liver surgery and transplantation and ischemic preconditioning is a promising strategy for improving the outcome of liver surgery. The preconditioning p...

Brief episodes of ischemia can protect against subsequent damaging ischemic events; however, the molecular mechanisms responsible for protection are poorly understood. Identifying genes involved in this process could provide insight into cell survival and treatment of stroke. We developed a murine model of ischemic preconditioning and subsequent stroke and used gene expression profiling to iden...

Wavelet methods allow to combine high order accuracy, eecient preconditioning techniques and adaptive approximation, in order to solve eeciently elliptic operator equations. Many diiculties remain, in particular related to the adaptation of wavelet decompositions to bounded domains with prescribed boundary conditions, as well as the possibly high constants in the O(1) preconditioning. In this p...

The present study was designed to investigate the possible role of free radicals in cardioprotective effects of ischemic, pharmacological and remote preconditioning. Isolated rat heart was perfused on Langendorff apparatus with Kreb's Henseleit solution and subjected to 30 min global ischemia followed by 120 min reperfusion. To assess myocardial injury, coronary effluent was analyzed for lactat...

Organisms have developed complex endogenous defenses to counter environmental stress. The successful resistance to adversarial conditions, such as calorie or oxygen deprivation, has ensured that the strongest will survive. This lies at the root of preconditioning, that is, what is survived will strengthen; surviving mild forms of injury leads to tolerance of otherwise noxious injury. Preconditi...