the aim of the present study was to investigate the effect of follicle stimulating hormone (fsh) on survival, activation and growth of ovine primordial follicles using histological studies. pieces of ovine ovarian cortex were obtained and cultured for 6 days in minimum essential medium supplemented with or without fsh (50 ng/ml). these fragments were then process to be used for histology compar...

Abstract Study question Could a hydrogel with similar mechanical properties of reproductive age ovarian cortex support the growth human preantral follicles? Summary answer Yes, our optimized PEGylated fibrin tailored strength provides suitable substrate for follicle survival and growth. What is known already The most challenging part assembling an engineered ovary developing appropriate scaffol...

Expression of pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide with considerable homology to vasoactive intestinal peptide, has been shown to be stimulated by gonadotropins in the ovary. The present studies further evaluated the cell-type specific expression and gonadotropin regulation of PACAP type I receptor (PACAPR) messenger RNA in immature rat ovaries and in cultu...

Background: Polycystic ovary syndrome (PCOS) is a heterogeneous disease characterized by hyperandrogenaemia, hirsutism, oligo-or amenorrhea, and anovulation. A common feature of PCOS is the accumulation of small subcortical follicles and increased ovarian stromal volume, causing a characteristic ultrasound image and a basis for the most commonly used designation for the syndrome. In order to th...

It has been shown that chronic exposure to cypermethrin (CPM), a pyrethroid pesticide, results in follicular atresia via pathologically affecting angiogenesis, disrupting endocrine potential and enhancing oxidative stress. This study was aimed to uncover the CPM-exposed energy dependent follicular cells apoptosis and to estimate protective effect of vitamin E (VitE) as a potent antioxidant. Thi...

Background: Ovarian cortex xenotransplantation into immunodeficient rats is a follicles protection method in rare animals and investigation on folliclogenesis. The aim of this study was to evaluate the effects of gonadotropin and erythropoietin (EPO) treatment on follicular development in the transplanted ovaries. Materials and Methods: Prepared sheep ovarian cortical strips were vitrified by c...

Introduction: Melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. This study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. Materials and Methods: Preantral follicles with diamet...