Pseudomonas aeruginosa is an opportunistic pathogen of particular significance to cystic fibrosis patients. This bacterium produces the exopolysaccharide alginate, which is an indicator of poor prognosis for these patients. The proteins required for alginate polymerization and secretion are encoded by genes organized in a single operon; however, the existence of internal promoters has been repo...

Preliminary studies by Beers et a2. (1) have shown that the zinc salt of acridine orange can either inhibit or accelerate the rate of polymerization of polynucleotides by polynucleotide phosphorylase of Micrococcus lysodeileticus, depending upon the experimental conditions. We have now confirmed these findings with the zinc-free dye. Some evidence for a reversal of inhibition with degraded ribo...

Preliminary studies by Beers et a2. (1) have shown that the zinc salt of acridine orange can either inhibit or accelerate the rate of polymerization of polynucleotides by polynucleotide phosphorylase of Micrococcus lysodeileticus, depending upon the experimental conditions. We have now confirmed these findings with the zinc-free dye. Some evidence for a reversal of inhibition with degraded ribo...

1. Multifunctional Initiators. 3749 2. Multifunctional Linking Agents 3751 3. Use of Difunctional Monomers 3754 B. Star−Block Copolymers 3754 C. Functionalized Stars 3755 1. Functionalized Initiators 3755 2. Functionalized Terminating Agents 3756 D. Asymmetric Stars 3757 1. Molecular Weight Asymmetry 3757 2. Functional Group Asymmetry 3760 3. Topological Asymmetry 3761 E. Miktoarm Star Polymers...

Controlled radical polymerization has proven to be a viable route to obtain polymers with narrow polydispersities (PDI’s) and controlled molecular weights under simple reaction conditions. It also offers control over the chain‐ends of the synthesized polymer. Atom transfer radical polymerization (ATRP) is the most studied and utilized of these techniques. In this study ATRP has ...

We have reexamined the interaction of purified platelet profilin with actin and present evidence that simple sequestration of actin monomers in a 1:1 complex with profilin cannot explain many of the effects of profilin on actin assembly. Three different methods to assess binding of profilin to actin show that the complex with platelet actin has a dissociation constant in the range of 1 to 5 mic...

In this research thin film heterogeneous cation exchange membrane was prepared by interfacial polymerization of polyacrylic acid-co-iron nickel oxide nanoparticle son PVC based substrate. Spectra analysis confirmed graft polymerization conclusively. The SEM images showed that polymerized layer covers the membranes by simple gel network entanglement. Results exhibited that membrane water content...

Abstract Chain polymerizations are defined as chain reactions where the propagation steps occur by reaction between monomer(s) and active site(s) on polymer chains with regeneration of at each step. Many forms polymerization can be distinguished according to mechanism step (e.g., cyclopolymerization – when rings formed, condensative is a condensation reaction, group-transfer polymerization, pol...

This paper shows that the protein-primed DNA polymerases encoded by bacteriophages Nf and GA-1, unlike other DNA polymerases, do not require unwinding or processivity factors for efficient synthesis of full-length terminal protein (TP)-DNA. Analysis of their polymerization activity shows that both DNA polymerases base their replication efficiency on a high processivity and on the capacity to co...

The GTPase Rac controls signaling pathways often related to actin polymerization in various cell types. In T lymphocytes, Rac is activated by Vav, a major component of the multiprotein transduction complex associated to the TCR. Although profound signaling defects have been observed in Vav-deficient mice, a role of Rac in the corresponding early TCR signaling has not been tested directly. This ...