BACKGROUND Hepatitis delta virus is a unique human pathogen responsible for some 20 million infections globally. This virus is dependent on hepatitis B virus for transmission and propagation. Currently, at least three genotypes of hepatitis delta virus with different geographic distribution and clinical manifestations are described. METHODS In this study, hepatitis delta virus RNA of 35 patie...

We present a fluorimetry-based technology for micro-RNA-21 (miR-21) sensing based on the concentration of miR-molecular beacon (MB) complexes and flushing unbound MB. This module consists microfluidic channel with shape funnel operated electrohydrodynamic actuation. report limit detection 2 pM in less than 1 min miR-21 alone, then demonstrate that levels, measured fine needle biopsy samples, fr...

Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are severe adverse cutaneous reactions to drugs. We describe the case of a 19 year old patient with SJS/TEN overlap syndrome, who developed severe interstitial pneumonia after she had received antiepileptic drugs. A cytomegalovirus infection was diagnosed by Real Time Polymerase Chain Reaction (RT-PCR) detection on Bronchoalveo...

Bovine viral diarrhoea virus (BVDV) is an important pathogen of dairy cattle. In this study, bulk milk samples representing a total of 4105 milking cows, from 18 dairy cattle herds in the suburb of Mashhad- Iran, were tested for presence of BVDV by the use of a nested reverse transcription polymerase chain reaction (Nested RT- PCR) assay. Non of the cows in the herds had been vaccinated against...

The hologic panther fusion (PF) platform provides fully automated CE marked diagnostics for respiratory viruses, including the recently discovered severe acute syndrome-coronavirus 2 (SARS-CoV-2) by a transcription mediated amplification (TMA) assay, but not endemic human coronaviruses (hCoV). Therefore, laboratory developed test (LDT) comprising multiplexed reverse polymerase chain reaction (R...

<p><strong>Background : </strong>The first case of COVID-19 in Indonesia was recorded March 2020. Limitation reverse-transcription polymerase chain reaction (RT-PCR) has put chest CT as an essential complementary tool the diagnosis and follow up treatment for COVID-19. Literatures strongly suggested that High-Resolution Computed Tomography (HRCT) is diagnosing typical symptoms...

Abstract Background The molecular mechanisms for chronic kidney disease (CKD) remain largely unknown and appear to be multifactorial. In the current study, we aimed study circulatory levels of circular ankyrin repeat domain 36 (circANKRD36) ANKRD36 in Egyptian patients with type 2 diabetes mellitus (T2DM) CKD explore their associations progression CKD. This cross-sectional controlled enrolled 6...

Duck spleen necrosis disease (DSND) caused by Novel Reovirus (NDRV), is an emerging infectious that causes severely threaten to duck industry. Currently, the popular conventional RT-PCR technique for detecting NDRV time consuming. So, it essential develop a rapid and accurate molecular diagnosis techniques of pathogen purpose effective control disease. In our study, simple, reliable detection m...

Objective: To determine the diagnostic value of rapid COVID-19 antigen test (Panbio, Abbott) in comparison with Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) suspected community cases SARS-CoV-2 infection at a tertiary care center Pakistan 
 Study Design: Cross sectional study.
 Place and Duration Study: Pathology Department, Combined Military Hospital, Lahore Pakistan, fro...

One of the viral diseases affecting both domestic and wild animals in many countries around world is foot mouth disease (FMD). The livestock sector has been suffering significant financial losses for years as a result disease. present research work was focused on isolation, documentation serotyping Foot Mouth Disease (FMD) virus from different locations. Reverse Transcription Polymerase Chain R...