conclusions simpleprobe® real-time pcr is a fast and accurate technique qualified to detect vkorc1 and cyp2c9 snps. these results encourage taking further steps towards using vkorc1 and cyp2c9 allelic screening to prevent clinical complications due to resistance or sensitivity to warfarin as well as anti-coagulant dose adjustment. results the allelic frequencies for vkorc1 c1173t (cc, ct, tt) w...

background: listeria monocytogenes is one of the most important food-borne bacteria causing septicemia, meningitis and encephalitis in humans. the objective of this study was to evaluate the occurrence and antibiotic resistance of the bacterium in retail minced beef in ahvaz, south-west of iran. methods: in this survey, 150 samples of minced beef were randomly obtained from retail butcheries in...

considering the importance of theileria equi infection in horse breeding industry and marketing, in the present study, we aimed to determine the prevalence of t. equi among dromedaries in central iran, where a considerable number of camels and horses are raised and equine theileriosis is quite prevalent. for this purpose, a total of 161 blood samples from camels were examined in terms of t. equ...

background: genetic polymorphism of milk proteins has been associated with composition, manufacture, and traits of milk. caseins are the most important milk proteins whose genes are strongly linked and inherited as a raceme. κ-casein which is a quantitatively minor constituent of bovine milk is thought to play a critical role in organization, fixation and aggregation of casein micelles and firm...

ornithobacterium rhinotracheale (ort) is a respiratory pathogen which has been isolated throughout the world from numerous bird species. the present study was designed to isolate and characterize the ort from domestic turkeys, quails and pigeons. for this purpose, 250 samples from each bird species (turkey, quail and pigeon) with or without respiratory signs were tested by taking of tracheal...

canine parvovirus type 2 (cpv-2) is one of the most common viruses responsible for acute hemorrhagic enteritis in dogs. a rapid and accurate diagnosis of cpv-2 infection is especially important in kennels in order to isolate infected dogs. the aim of the present study was to compare two laboratory tests i.e., polymerase change reaction (pcr) and immunochromatography assay (ica) most commonly us...

results among the 181 anti-hcv ab negative patients, 13 (7.2%) were positive for hcv core ag and 11 (6%) had detectable hcv rna with a range of 40-336543 iu/ml by pcr. the two tests had a high measurement agreement (kappa=0.82, p<0.001). of the 13 patients with positive hcv core ag test results, 3 were negative for hcv rna. considering real time pcr for hcv rna as the gold standard for hcv infe...

conclusions the quite sensitive in-house taqman real time rt-pcr assay was able to detect and quantify all four main hcv genotypes prevailing around all geographical regions of iran. results the lower limit detection of this in-house hcv real-time rt-pcr was determined as 100 rna copies/ml. inter- and intra-assay coefficient of variation (cv) of this in-house hcv real-time rt-pcr ranged from 0....

background and aims: influenza virus is the most important cause of annual morbidities and mortalities worldwide with numerous antigenic drifts and shifts. inaccessibility to effective drugs and vaccines has made world health authorities to be interested in traditional medicine in order to prevent spread of the infectious agent. garlic is one of the most famous of all plants in human history. i...