although mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. to address this limitation, the authors developed and optimized species-specific primer pairs that target m. eguigenitalium rpob (rna polymerase b subunit) gene sequences. the specificity of the pcr assay developed in this stu...

background and objective: human papillomavirus (hpv) is one of the possible etiologic factors  in development of esophageal squamous cell carcinoma (escc). in this study we aimed to study the  role of hpv in escc. patients and methods:in this study, 140 cases of escc were analyzed for the hpv dna by  polymerase chain reaction (pcr) using gp5+/gp6+ primers for l1 open reading frame (orf) to  am...

background: considering that there was not any regional survey in isfahan, iran regarding the epidemiology of acute respiratory tract infections (arti) in different age groups of general population, the aim of this study was to determine the epidemiologic feature of artis in isfahan using multiplex polymerase chain reaction (pcr) method. materials and methods: in this cross-sectional study,pati...

introduction: methicillin-resistant staphylococcus aureus (mrsa) can cause serious and life-threatening hospital- and community-acquired infections. colonized and infected patients represent the most important reservoir of mrsa in health care facilities. therefore, in this study, mrsa isolates collected from shohada hospital in tabriz were evaluated for the frequency of meca gene and their anti...

background: sarcocystosis in south american camelids (sac) is an important parasitic disease which results in economical loss due to carcass condemnation. meat products from camelids are significant source of animal protein in several american countries. sarcocystis spp. producing macroscopical cysts in these animals have been nominated as s. aucheniae, s. tilopodi, and s. guanicoecanis. the ai...

background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...

objective(s) this study planned to assess the value of pcr is6110 assay in tissue specimens of needle pleural biopsy in patients suspicious to pleural tuberculosis. materials and methods sixty eight patients with lymphocytic exudative pleural effusion underwent pleural biopsy. tissue samples were sent for pathologic examination and pcr is6110 assay. the results of pcr reported as positive/ nega...

three tuberculous, twenty-one non-tuberculous mycobacterial (ntm) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of dna fragments in a polymerase chain reaction with single universal primer (up-pcr). a rather wide limit of tolerance for variations in procedure of pcr mixture preparation and thermocycling parameters was found. there...

purpose : the evaluation of the presence of herpes simplex virus (hsv) in corneal scars by real-time polymerase chain reaction (pcr) and comparison of the results with histopathologic findings and clinical diagnoses methods : eighty-seven corneal scar samples obtained after penetrating keratoplasty (pk), were selected after reviewing the records from 2006 to 2010. nucleic acid was extracted fro...