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Polymer gels have been shown to behave as viscoelastic materials but only a small amount of data is usually provided in the glass transition. In this paper, the dynamic moduli G′ and G′′ of polyacrylamide hydrogels are investigated using both an AFM in contact force modulation mode and a classical rheometer. The validity is shown by matching of the two techniques. Measurements are carried out o...

Vertical polyacrylamide gel electrophoresis (PAGE) is routinely used in molecular biology to separate macromolecules. DNA sequencing, singlestranded conformational polymorphism (SSCP) analysis and microsatellite typing all utilize the high resolution capabilities of polyacrylamide gels to separate molecules that differ in size by as little as one base pair. However, preparation of these gels is...

A new scanning probe-based microrheology approach is used to quantify the frequency-dependent viscoelastic behavior of both fibroblast cells and polymer gels. The scanning probe shape was modified using polystyrene beads for a defined surface area nondestructively deforming the sample. An extended Hertz model is introduced to measure the frequency-dependent storage and loss moduli even for thin...

Herpes simplex virus, grown in BHKzI cells, was extensively purified by fluorocarbon treatment, ultracentrifugation, sucrose gradient centrifugation and chromatography on calcium phosphate. The purity of the product was assessed by immunodiffusion, electrophoresis in polyacrylamide gels and removal of added radioactive host material from the virus. Purified virus was disrupted with sodium dodec...