Nowadays, there is an increasing interest toward the substitution of oil-based polymers with biobased polymers. Poly(hydroxyalkanoates) (PHAs) emerge as a new class of biopolymers, that have gained more and more attention due to their thermoplastic behaviour, acceptable mechanical properties and biodegradability in compost conditions1. Applications based on PHAs, as a matrix, are generally foun...

2 Dipartimento di Biotecnologie Agrarie, Universita di Padova, Via Gradenigo 6, 35131 Padova, Italy Genes encoding fiketothiolase (phaA), acetoacetyl-CoA reductase (phaB) and PHB-synthase (phaC) from R. meliloti 41, together with a fourth gene, referred to as ORF1, presumed to be involved in PHB biosynthesis, have been cloned and sequenced. phaA, phaB and ORFl were identified by heterologous hy...

Rosin natural terpene (Dertoline) is compounded with a bacterial thermoplastic polyesters polyhydroxyalkanoate (PHA), specifically here poly(3-hydroxybutyrate) (PHB), biodegradable and biocompatible polymer. The compatibility of the blend examined differential scanning calorimetry thermal gravimetry analysis. In range concentration Dertoline, from 5 to 15 wt%, cold crystallization peak first sh...

Poly-3-hydroxybutyrate (PHB) is a natural polymer synthesized by many bacteria as a carbon-energy storage material. It was accumulated maximally prior to the spore formation but was degraded during the process of sporulation in Bacillus thuringiensis. Intriguingly, B. thuringiensis also accumulates large amounts of insecticidal crystal proteins (ICPs) during sporulation, which requires consider...

This study investigated the protective effect of poly-β-hydroxybutyrate (PHB) on Chinese mitten crab, Eriocheir sinensis, zoea larvae challenged with pathogenic Vibrio anguillarum. PHB was delivered to the crab larvae through rotifer and Artemia bioencapsulation. Zoea 3 larvae were challenged with V. anguillarum at a final concentration of 10(5) CFU mL(-1). PHB-enriched rotifers and Artemia nau...

Background: In the present study, we aim to utilize the ecological diversity of soil for the isolation and screening for poly β-hydroxybutyrate (PHB)-accumulating bacteria and production of cost-effective bioplastic using cardboard industry effluent. Results: A total of 120 isolates were isolated from different soil samples and a total of 62 isolates showed positive results with Nile blue A sta...

Methanotrophic-heterotrophic communities were selectively enriched from sewage sludge to obtain a mixed culture with high levels of poly-β-hydroxybutyrate (PHB) accumulation capacity from methane. Methane was used as the carbon source, N2 as sole nitrogen source, and oxygen and Cu content were varied. Copper proved essential for PHB synthesis. All cultures enriched with Cu could accumulate high...

The carbon that rhizobia in root nodules receive from their host powers both N(2) fixation, which mainly benefits the host, and rhizobium reproduction. Rhizobia also store energy in the lipid poly-3-hydroxybutyrate (PHB), which may enhance rhizobium survival when they are carbon limited, either in nodules or in the soil between hosts. There can be a conflict of interest between rhizobia and leg...

The effect of different growth conditions on the glycogen and poly-/I-hydroxybutyrate (PHB) content of the cyanobacterium Spirulina maxima is described. Under photoautotrophic growth conditions without any nutrient limitation, S. maxima exhibited a glycogen content of between 7-1 and 10.7% of cell dry wt, whereas PHB was undetectable. When S. maxima was grown under mixotrophic conditions in the...