Adult pluripotent stem cells have been presented as an alternative in the stem cells research to replace the utilization of embryonic stem cells which pose ethical dilemma. The possibility of harvesting adult pluripotent stem cells for medical purposes resulted in a growing interest in stem cell research. In particular, researchers need to identify differentiation state of stem cells in a fast ...

E-cadherin; pluripotency; embryonic stem cell; induced pluripotent stem cell; iPS; ES; signaling pathways; Activin; Nodal.

The unexpected discovery that somatic cells can be reprogrammed to a pluripotent state yielding induced pluripotent stem cells has made it possible to produce cardiovascular cells exhibiting inherited traits and disorders. Use of these cells in high throughput analyses should broaden our insight into fundamental disease mechanisms and provide many benefits for patients, including new therapeuti...

Low efficiency of somatic cell reprogramming and heterogeneity among human induced pluripotent stem cells (hiPSCs) demand extensive characterization of isolated clones before their use in downstream applications. By monitoring human fibroblasts undergoing reprogramming for their morphological changes and expression of fibroblast (CD13), pluripotency markers (SSEA-4 and TRA-1-60) and a retrovira...

Since the first studies of human embryonic stem cells (hESCs) and, more recently, human induced pluripotent stem cells (hiPSCs), the stem-cell field has been abuzz with the promise that these pluripotent populations will one day be a powerful therapeutic tool. Although it has been proposed that hiPSCs will supersede hESCs with respect to their research and/or clinical potential because of the e...

A Need for meat is rising day by and the production of poultry, cows, pigs fisheries reduced as there no good commercial production. Wider livestock becoming unavailable on a global scale in requisites welfare animals, ecological sustenance human health conditions. Choices cultured are being explored using innovative techniques like stem cells, Embryonic Stem Cells, Induced pluri potent cells [...

Induced pluripotent stem (iPS) cells, are a type of pluripotent stem cell derived from adult somatic cells. They have been reprogrammed through inducing genes and factors to be pluripotent. iPS cells are similar to embryonic stem (ES) cells in many aspects. This review summarizes the recent progresses in iPS cell reprogramming and iPS cell based therapy, and describe patient specific iPS cells ...

Recent work from our group and others has argued that human induced pluripotent stem cells (hiPSCs) generated by the introduction of four viruses bearing reprogramming factors differ from human embryonic stem cells (hESCs) at the level of gene expression (Chin et al., 2009). Many of the differences seen were common across independent labs and, at least to some extent, are thought to be a result...

Pluripotency is generally defined by the ability to differentiate into cell types representing all three germ layers: ectoderm, mesoderm, and endoderm. Human pluripotent stem cells hold great promise in regenerative medicine and in cell replacement therapies because of their ability to self-renew and their developmental potential to become all cell types in the body. Moreover, pluripotent cells...

Ectopically, expression of defined factors could reprogram mammalian somatic cells into induced pluripotent stem cells (iPSCs), which initiates a new strategy to obtain pluripotent stem cell lines. Attempts have been made to generate buffalo pluripotent stem cells by culturing primary germ cells or inner cell mass, but the efficiency is extremely low. Here, we report a successful method to repr...