Surface sterilization of explant is an important and most sensitive step in plant tissue culture. Inappropriate concentrations of sterilants have lethal effect in cell division and it restricts growth and development of explant. Therefore, suitable concentration, combinations and duration of exposure of sterilant is essential to raise in vitro cultures successfully. This data demonstrates use o...

Tissue culture is an in-vitro technique for replicating a plant without introducing the degree of genetic variability inherent in sexual reproduction. Tissue culture has proven commercially important for the replication of ornamental varieties of house plants and is used extensively in research for applications in forestry. Activated carbon (AC) was initially added to tissue culture media in an...

Embryogenic callus was obtained from mature seed explants on medium supplemented with 2,4-dichlorophenoxyacetic acid. Primary somatic embryos (SEs) can only develop into abnormal plants. Well-developed SEs could be obtained through secondary somatic embryogenesis both in solid and liquid cultures. Temperature strongly affected induction frequency of secondary embryogenesis. Relatively high temp...

This study describes the standardization of an efficient in vitro propagation and hardening procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings were germinated on half-strength 1/2 MS medium supplemented with 10 g·Lsucrose, 5.5 g·Lagar at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·LTDZ, 0.1 mg·LNAA supported the maximal auxil...

Different explants of Crocus sativus L. such as corm, style, ovary, petal, anther and leaf were cultured on LS and MS media supplemented with NAA and BAP or 2,4-D plus BAP at different combinations. Shoot and corm induction occurred in addition to the formation of other organs. Efficient plantlet regeneration with strong roots occurred at 1 mg 1-I of both NAA and BAP. An increase in 2,4-D l...

in this study, physiological and morphological responses of five almond cultivars to drought stress were investigated under in vitro conditions. plantlets from five commercial almond cultivars (supernova’, ‘tuono’, ‘sahand’, ‘ferragnes ’ and ‘shahroud 21’] were established in ms medium containing 0.5 mg per liter bap and then subcultured  in ms proliferation medium containing 1 mg per liter bap...

to evaluate the allelopathic effect of essential oil extracted from leaves of eucalyptus globules against some certain weeds and the possibility of that being employed in weed management strategies, laboratory and greenhouse studies were conducted in islamic azad university of khoy in 2007 in the framework of a factorial expreiment based on a randomized complete block design of four replication...

elimination of potyviruses from two commercial iranian garlic populations, hamedan and azar-shahr, through thermotherapy and by meristem tip culture were investigated. the infected bulbs of either one of the populations were initially treated at 30o c for one week and then at 36oc for 5 weeks. the meristems of cloves at two sizes of 0.4-0.6 mm and 0.6-0.8 mm were then cultured on ms medium supp...