objective(s):human cytomegalovirus (hcmv) remains a major morbidity and mortality cause in immuno suppressed patients. therefore, significant effort has been made towards the development of a vaccine. in this study, the expression of the pp65 and gb fusion peptides and fc domain of mouse igg2a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (apcs) in pich...

Members of the six-transmembrane segment family of ion channels share a common structural design. However, there are sequence differences between the members that confer distinct biophysical properties on individual channels. Currently, we do not have 3D structures for all members of the family to help explain the molecular basis for the differences in their biophysical properties and pharmacol...

Myostatin propeptide can inhibit the biological activity of myostatin protein and promote muscle growth. To express myostatin propeptide in vitro with a higher biological activity, we performed codon optimization on the sheep myostatin propeptide gene sequence, and mutated aspartic acid-76 to alanine based on the codon usage bias of Pichia pastoris and the enhanced biological activity of myosta...

−We established a gene expression process to produce a flavor peptide in Pichia pastoris. The octapeptide Lys-Gly-Asp-Glu-Glu-Ser-Leu-Ala was isolated from beef digested by papain, known as “beefy meaty peptide” (BMP). It was considered a savory seasoning in previous literatures. To produce BMP by microorganisms, BMP expression structures encoding tandem repeats of the octapeptide were designed...

The surfaces of grapes are covered by different yeast species that important in the first stages fermentation process. In recent years, non-Saccharomyces yeasts such as Torulaspora delbrueckii, Lachancea thermotolerans, Metschnikowia pulcherrima, and Pichia kluyveri have become popular with regard to winemaking improved wine quality. For reason, several manufacturers started offer commercially ...

Abstract. Planonth S, Chantarasiri A. 2022. The oleaginous yeast Pichia manshurica isolated from Lansium domesticum fruit in Thailand and its fatty acid composition of single cell oil. Biodiversitas 23: 801-809. Oleaginous microbes can accumulate intracellular lipids or oils (SCOs) quantities higher than 20% their biomass. They be a sustainable alternative to fossil fuels, biofuels, oleochemica...

Em 1877 Connheim descreveu pela primeira vez o mecanismo de embolia paradoxal através do forâmen ovale patente (FOP), fenómeno múltiplas vezes documentado em autópsias e estudos ecocardiográficos. Além da migração de um trombo através do defeito septal, outros mecanismos têm sido propostos para implicar o FOP em fenómenos cardioembólicos, nomeadamente um aumento da vulnerabilidade auricular a a...

The third level is not properly linguistic, but serves as a tertium comparationis for linguistic strategies analyzed at the two lower levels. For example, in the German sentence Rita schnitt dem Kind die Haare 'Rita cut the child's hair', the NP dem Kind is a certain kind of adjunct (dativus sympatheticus) at level 1, a benefactive adjunct at level 2 and both a beneficiary and a possessor at le...