To develop apple proliferation (AP) resistant rootstocks, a breeding program was initiated in 2001 employing apomictic Malus sieboldii and M. sieboldii-derived hybrids as donors of the resistance trait and mainly standard apple rootstock Malus x domestica cv. M9 as donor of agronomic values. Examination of the experimentally inoculated progeny of seven crossings made in 2001 and 2002 over a per...

Phytoplasma-associated diseases are reported for more than 1,000 plant species worldwide. Only a few genome sequences are available in contrast to the economical importance of these bacterial pathogens. A new strategy was used to retrieve phytoplasma strain-specific genome data. Multiple displacement amplification was performed on DNA obtained from <3 g of plant tissue from tobacco and parsley ...

Phytoplasma is an insect-transmitted pathogen that causes witches' broom disease in many plants. Paulownia witches' broom is one of the most destructive diseases threatening Paulownia production. The molecular mechanisms associated with this disease have been investigated by transcriptome sequencing, but changes in protein abundance have not been investigated with isobaric tags for relative and...

Phytoplasmas associated with cucumber phyllody (CuP) and squash phyllody (SqP) in Yazd province of Iran were characterized by molecular analyses and biological studies. Orosius albicinctus leafhoppers testing positive for phytoplasma presence by polymerase chain reaction (PCR) successfully transmitted CuP and SqP phytoplasmas to healthy cucumber and squash plants. The phytoplasmas were also tra...

Paulownia fortunei is a widely cultivated economic forest tree species that is susceptible to infection with phytoplasma, resulting in Paulownia witches' broom (PaWB) disease. Diseased P. fortunei is characterized by stunted growth, witches' broom, shortened internodes, and etiolated and smaller leaves. To understand the molecular mechanism of its pathogenesis, we applied isobaric tags for rela...

Walnut trees displaying symptoms similar to those caused by phytoplasmas, including foliar chlorosis, yellowing and shortening of internodes, were found in a fruit orchard the north Fars province, Iran 2020 (Fig. 1). Samples collected analysed PCR detect identify possible phytoplasma(s) associated with diseased plants. Four four asymptomatic walnut sampled for presence phytoplasma. Total DNA wa...

Numerous yellows-type diseases of plants were once thought to be caused by viruses in view of their infective spread, their symptomatology, and the fact that they were transmitted by insects. The first demonstration that the etiological agents of yellows diseases could be wall-less prokaryotes rather than viruses caught the field of plant pathology by surprise more than forty years ago (Doi et ...

During the autumn seasons of 2016-2019, several surveys were carried out in grapevine (Vitis vinifera L.) cultivation areas ?anl?urfa and Ad?yaman provinces South-eastern part Turkey, to identify plants exhibiting phytoplasma-like symptoms including yellowing, small leaf formation, chlorosis, short internodes, severe redness inward curling detect characterize causal agent responsible for these ...

Phytoplasma strains were sporadically found in specimens of Hishimonus hamatus, Japananus hyalinus and Graphocephala fennahi caught during an entomological survey conducted Southern Switzerland on the edges woods surrounding cultivated vineyards 2017. The subsequent molecular characterization pathogens allowed to identify them as strictly associated Flavescence dorée, a serious European grapevi...

‘Flavescence dorée’ is a serious phytoplasma disease affecting grapevine in several European countries. We are currently studying the interaction of ‘flavescence dorée’ phytoplasma with its natural plant host, by monitoring the effects of infection on the protein expression profile. The red-berried V. vinifera cv. Nebbiolo and Barbera were the source of plant material, as they are widely grown ...