Abstract Background The present work is aimed at development and validation of RP HPLC method which simple, specific, precise, accurate for estimation Sofosbuvir its process-related impurity in bulk pharmaceutical dosage forms. Extensive literature survey revealed no the above said. chromatographic separation was achieved on Agilent Eclipse XDB-C18, 4.6 × 250 mm, 5 μm with mobile phase composed...

A new type of nucleic acid analogues with a phosphoryl guanidine group is described. Oxidation of polymer-supported dinucleoside 2-cyanoethyl phosphite by iodine in the presence of 1,1,3,3-tetramethyl guanidine yields a dinucleotide with an internucleoside tetramethyl phosphoryl guanidine (Tmg) group as the main product. The Tmg group is stable under conditions of solid-phase DNA synthesis and ...

The second-order rate constants for reaction of the Mg2+ complexes of phosphorylated pyridine monoanions with Mg(OH)+ a re 104-106-fold larger than the second-order rate constants for their reaction with water (25 OC, ionic strength 1.5). Of the 106-fold rate enhancement with the phosphorylated 4morpholinopyridine/Mg2 complex, 1 04-fold is attributed to the greater nucleophilicity of Mg(OH)+ co...

Tryptic digestion of the diisopropyl phosphoryl-32P-Scarboxymethyl light chain derivative of human plasmin followed by gel filtration on Sephadex G-75 and G-25 and chromatography on Dowex SOW-X2 has resulted in the isolation of three radioactive peptides. These three peptides, having 31,33, and 34 residues, are all derived from the same segment of the plasmin molecule. Structural determinations...

1. The phosphorylation of milk alkaline phosphatase was studied under various conditions: maximum incorporation occurred at pH5.0 and 50% incorporation at pH6.6-7.0. 2. The phosphorylation was shown to be specific and the results suggest that the active centre of the enzyme is involved in the process. 3. Phosphoryl-enzyme is rapidly hydrolysed at alkaline pH. at pH7.0 the results suggest that a...

As part of a study on enzymic catalysis of group transfer, this laboratory has previously reported on the enzymic transfer of the glucuronyl radical from a variety of donor substrates to a number of acceptor alcohols (1). The phenomenon was studied in detail and conditions for efficient group transfer were investigated. The present paper reports the use of a similar approach in studying the aci...